留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码
吴康, 薛明强, 吴海琴, 李义, 宋学宏, 王裕兴. 鲤血管内皮细胞的分离培养及初步鉴定[J]. 水生生物学报, 2001, 25(2): 174-178.
引用本文: 吴康, 薛明强, 吴海琴, 李义, 宋学宏, 王裕兴. 鲤血管内皮细胞的分离培养及初步鉴定[J]. 水生生物学报, 2001, 25(2): 174-178.
WU Kang, XUE Ming-qiang, WU Hai-qin, LI Yi, SONG Xue-hong, WANG Yu-xing. SEPARATION CULTURE AND PRELIMINARY IDENTIFICATION OF THE ENDOTHELIAL CELL OF CYPRINUS CARPIO[J]. ACTA HYDROBIOLOGICA SINICA, 2001, 25(2): 174-178.
Citation: WU Kang, XUE Ming-qiang, WU Hai-qin, LI Yi, SONG Xue-hong, WANG Yu-xing. SEPARATION CULTURE AND PRELIMINARY IDENTIFICATION OF THE ENDOTHELIAL CELL OF CYPRINUS CARPIO[J]. ACTA HYDROBIOLOGICA SINICA, 2001, 25(2): 174-178.

鲤血管内皮细胞的分离培养及初步鉴定

SEPARATION CULTURE AND PRELIMINARY IDENTIFICATION OF THE ENDOTHELIAL CELL OF CYPRINUS CARPIO

  • 摘要: 鲤动脉球腔内灌注0.1%胶原酶消化,分离到大量内皮细胞及少量成纤细胞,28℃,在加入蛋白含量为:350μg/mL鲤下丘脑粗提液的 1640+20%小牛血清培养液中,细胞生长良好,7d后,接近单层.原代经三次 0.5%柠檬酸胰酶消化逐步淘汰少量成纤维细胞,10d后,得纯内皮细胞,并顺利传至二代,第二代细胞经血凝ⅧR因子酶标检测发现,培养细胞存在血凝ⅧR因子相同抗原,具有内皮细胞的一般特征,从而得到初步鉴定.

     

    Abstract: Many endothelial cells and few fibro-cells were separated from the artery ball Cyprinus carpio injected with 0.1% collagense(I). At 28, in the 1640+10% calf bovine serum medium which was supplemented with 350μg/mL carp hypothulumus-derived growth substance, these cells grew well and the cell monolayers were obtained within 7-days period. By three times digestion of 0.5% trypsin-citric acid solution, the pure endothelial cells were gained after 10 days and they were subcultured to which. The second generation were proved to have VIIIR factor antigen by anti-VIIIR factor assay. These cells were identictied with general biological character of endothelial cells.

     

/

返回文章
返回