转兔防御素基因小球藻异养培养的培养基优化研究
OPTIMIZATIONOF HETEROTROPHICMEDIUM FOR CULTURING TRANSGENIC CHLORELLA WITH RABBIT DEFENSIN GENE
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摘要: 在氮源种类筛选的基础上,采用Plackett-Burman实验设计并结合单因素实验对转兔防御素基因小球藻异养培养的培养基进行了优化。实验结果表明,采用优化后的Knop异养培养基在250mL摇瓶中进行异养培养,藻细胞密度从优化前的1.5g/L提高到优化后的5.11g/L;摇瓶和5L生物反应器异养培养表明,Knop异养培养基中藻细胞浓度分别为优化前培养基的3.39倍和3.17倍,而兔防御素(NP-1)表达能力基本不变。Abstract: Based on the experiment of screening nitrogen sources, mixotrophicKnop mediumwas optimized by using of Plackett-Burman experimental design and the one-at-a-time strategy and the cell density of transgenic Chlorella was improved from 1. 12g/ L to 5. 11g/Lin 250mLflasks. Using the optimized medium, the cell density of the transgenic Chlorella in 250 mL flasks and 5L bioreactorwere 3. 39 and 3. 17 fold respectivelywhat can beobtained in themixotrophic Knop mediumwhile the expression capa-bilities of NP-1 were kept unchanged.
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