留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码
方平, 李英文, 胡炜, 丁诗华. 黄鳝cGnRH-Ⅱ的cDNA克隆与序列分析[J]. 水生生物学报, 2007, 31(2): 259-264.
引用本文: 方平, 李英文, 胡炜, 丁诗华. 黄鳝cGnRH-Ⅱ的cDNA克隆与序列分析[J]. 水生生物学报, 2007, 31(2): 259-264.
FANG Ping, LI Ying-Wen, HU Wei, DING Shi-Hua. CLONING AND SEQUENCE ANALYSIS OF cDNA ENCODING cGnRH-II FROM RICE FIELD EEL(MONOPTERUS ALBUS)[J]. ACTA HYDROBIOLOGICA SINICA, 2007, 31(2): 259-264.
Citation: FANG Ping, LI Ying-Wen, HU Wei, DING Shi-Hua. CLONING AND SEQUENCE ANALYSIS OF cDNA ENCODING cGnRH-II FROM RICE FIELD EEL(MONOPTERUS ALBUS)[J]. ACTA HYDROBIOLOGICA SINICA, 2007, 31(2): 259-264.

黄鳝cGnRH-Ⅱ的cDNA克隆与序列分析

CLONING AND SEQUENCE ANALYSIS OF cDNA ENCODING cGnRH-II FROM RICE FIELD EEL(MONOPTERUS ALBUS)

  • 摘要: 促性腺激素释放激素(Gonadotropin-releasing hormone,GnRH)是一个保守的十肽神经家族激素,在脊椎动物的性腺发育和繁殖功能的维持方面起着重要的调控作用。本文通过运用RACE和RT-PCR方法,从黄鳝脑组织中克隆得到cGnRH-ⅡcDNA全序列,其核苷酸序列长度为617bp。该cDNA编码的cGnRH-Ⅱ的前体氨基酸序列结构组成与其他物种的cGnRH-Ⅱ前体结构一致,其推导的蛋白前体长度为83个氨基酸,包括一个信号肽、cGnRH-Ⅱ十肽和一个由蛋白水解位点(Gly-Lys-Arg)连接的GnRH联接肽,其中信号肽和联接肽的长度分别为21和49个氨基酸。cGnRH-Ⅱ的氨基酸序列和其他相关物种cGnRH-Ⅱ氨基酸序列比较结果显示,cGnRH-ⅡcDNA的蛋白编码区高度保守,而非编码区的保守性程度很低。

     

    Abstract: Gonadotropin-releasing hormone (GnRH) is a conserved nerval decapeptidethat regulates the development and maintenance of reproductive function in vertebrate1The cDNA encoding the His5Trp7Tyr8 gonadotropin-releasing hormone ( chicken-?type GnRH, cGnRH-II) precursor from the brain of rice field eel ( Monopterus albus) has been isolated and sequenced by using reverse transcription and rapid amplification of cDNA ends ( RACE) 1From the subcloning and sequence analysis of 3c-RACE and 5c-RACE product, the complete sequence of the cDNA encoding its cGnRH-II peptide was identified and determined to be 617 bp in length1The structure of the encoded peptide is identical to all other cGnRH-II reported to date. The deduced peptide is composed of a signal peptide (SP), a GnRH decapeptide and a GnRH-associated peptide ( GAP) which is connected to GnRH by a Gly-Lys-Arg sequence1The cGnRH-II cDNA encoded SP of 21 amino acids and GAP of 49 amino acids. The amino acid sequence of cGnRH-II in combining with the Gly-Lys-Arg sequence is highly conserved during evolution when compared with the corresponding regions of other GnRH precursors. However, the untranslated region of GnRH are markedly divergent in nucleotide sequences

     

/

返回文章
返回