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沈宏, 林俊, 郑振华, 丁力, 周培疆. 有机磷农药对铜绿微囊藻生长及摄磷效应的动力学研究[J]. 水生生物学报, 2004, 28(2): 174-179.
引用本文: 沈宏, 林俊, 郑振华, 丁力, 周培疆. 有机磷农药对铜绿微囊藻生长及摄磷效应的动力学研究[J]. 水生生物学报, 2004, 28(2): 174-179.
SHEN Hong, LIN Jun, ZHENG Zhen-Hua, DING LI, ZHOU Pei-Jiang. KINETIC STUDIES ON THE EFFECTS OF ORGANOPHOSPHORUS PESTICIDES ON THE GROWTH OF MICROCYSTIS A. AND ITS UPTAKE TO PHOSPHORUS[J]. ACTA HYDROBIOLOGICA SINICA, 2004, 28(2): 174-179.
Citation: SHEN Hong, LIN Jun, ZHENG Zhen-Hua, DING LI, ZHOU Pei-Jiang. KINETIC STUDIES ON THE EFFECTS OF ORGANOPHOSPHORUS PESTICIDES ON THE GROWTH OF MICROCYSTIS A. AND ITS UPTAKE TO PHOSPHORUS[J]. ACTA HYDROBIOLOGICA SINICA, 2004, 28(2): 174-179.

有机磷农药对铜绿微囊藻生长及摄磷效应的动力学研究

KINETIC STUDIES ON THE EFFECTS OF ORGANOPHOSPHORUS PESTICIDES ON THE GROWTH OF MICROCYSTIS A. AND ITS UPTAKE TO PHOSPHORUS

  • 摘要: 研究了两种有机磷农药(甲胺磷、辛硫磷)在较低浓度时对铜绿微囊藻的生长效应以及微囊藻摄取磷形态:总溶解磷(TSP)、溶解反应磷(SRP)和溶解有机磷(DOP)的动力学规律.保持各培养基中总磷的浓度6.95mg/L,与培养基中不加有机磷农药相比,当培养基中甲胺磷与K2HPO4的浓度比为1:11和1:9,辛硫磷与K2HPO4的浓度比为1:349和1:114时,微囊藻的生长受到促进,并且当甲胺磷与K2HPO4,的浓度比为1:9,辛硫磷与K2HPO4的浓度比为1:114时促进效果最好,而当培养基中没有K2HPO4甲胺磷作为惟一磷源时,微囊藻的生长受到明显的抑制.微囊藻摄取SRP的速度快于其他磷形态,而对DOP的利用较少.微囊藻生长越快,对SRP的摄取速率也越快.结果表明低浓度有机磷农药促进微囊藻生长的主要原因是有机磷的加入促进了藻类对SRP的摄取.

     

    Abstract: Organophosphorus pesticides (OPs) are widely used in agriculture and they do not only kill target organisms but also extend to some nontarget organisms. Major portions of some nontarget organisms are algae cells. The objective of this work was to study the effects of low concentration methamidophs(MAP) and phoxime on the growth of Microcystis aeruginosa. Meanwhile, the kinetic mechanism of uptake of three kinds of phosphorus forms-total soluble phosphorus (TSP), soluble reaction phosphorus (SRP) and dissolved organic phosphorus (DOP) by Microcystis were also studied. The concentration of total phosphorus was 6.95 mg/L in each medium. Methamidophs and phoxime were diluted with sterile distilled water and added into the sterile modified HGZ medium at various phosphorus concentration ratios of methamidophs and phoxime to K2HPO4. Microcystis cell density measured by spectrophotometer every 24hr at 663nm after inoculation. And adopted ammonium molybdate spectrophotometric methods to determine the phosphorus forms concentration. Compared with no MAP and phoxime in medium, when MAP, phoxime and K2HPO4 combined in concentration ratio (1:11 and 1:9, 1:349 and 1:114) was treated, the growth of Microcystis was stimulated. And the algal cells in MAP, phoxime:K2HPO4 ratio 1:9,1:114 treated medium grew faster than that of in MAP:K2HPO4 ratio 1:11,1:349 treated medium. The growth of Microcystis significantly inhibited by MAP when no K2HPO4 in medium and MAP as unique P source of algal growth. The kinetic curves of change of phosphorus forms concentration in each medium were fit to logistic model. The SRP could be assimilated by algae at higher velocity than that of other phosphorus forms, and few DOP used by algae. The algal grew faster, the values of uptake velocity of the SRP by Microcystis were bigger. All these results showed that the MAP and phoxime could stimulate growth of Microcystis when they and inorganic phosphorus combined in certain ratio, but the point of the certain ratio for MAP is higher than that for the phoxime. The growth rate of Microcystis was dependent upon the concentration of intracellular phosphorus, and SRP was the preferential phosphorus form used by algae. The cause of stimulation might be the MAP and phoxime could accelerate the velocity of SRP uptake by the algal at the low concentration.

     

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