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潘黔生, 方之平, 廖瑞林. 四种鲤科鱼肠道中高血糖素免疫活性内分泌细胞的研究[J]. 水生生物学报, 1991, 15(1): 57-62.
引用本文: 潘黔生, 方之平, 廖瑞林. 四种鲤科鱼肠道中高血糖素免疫活性内分泌细胞的研究[J]. 水生生物学报, 1991, 15(1): 57-62.
Pan Qiansheng, Fang Zhiping, Liao Ruilin. STUDY ON GLUCAGON-IMMUNOREACTIVE ENDOCRINE CELLS IN THE GUT OF FOUR CYPRINID FISHES[J]. ACTA HYDROBIOLOGICA SINICA, 1991, 15(1): 57-62.
Citation: Pan Qiansheng, Fang Zhiping, Liao Ruilin. STUDY ON GLUCAGON-IMMUNOREACTIVE ENDOCRINE CELLS IN THE GUT OF FOUR CYPRINID FISHES[J]. ACTA HYDROBIOLOGICA SINICA, 1991, 15(1): 57-62.

四种鲤科鱼肠道中高血糖素免疫活性内分泌细胞的研究

STUDY ON GLUCAGON-IMMUNOREACTIVE ENDOCRINE CELLS IN THE GUT OF FOUR CYPRINID FISHES

  • 摘要: 应用过氧化物酶抗过氧化物酶(PAP)免疫组织化学技术,用4种哺乳动物中培育出的抗血清对草鱼、青鱼、鲤和翘嘴红鲌4种鲤科鱼的肠内分泌细胞进行免疫、组织化学的鉴别和定位.证实了高血糖素免疫活性内分泌细胞在草鱼整个肠道中均呈阳性反应;在鲤、青鱼前肠中仅有少量阳性反应;在翘嘴红鲌肠道中未见阳性反应。胃蛋白酶原(Pepsinogen)、凝乳酶(Prochymosin)和神经特异烯醇酶(Neuron specific enolase)3种抗血清在4种鱼的肠道中均未发现阳性反应.本文重点描述草鱼高血糖素免疫活性内分泌细胞在肠道各段的分布,密度及其形态学特征,还就其可能的生理功能与草食性哺乳动物胃肠道中该类内分泌细胞进行比较和讨论。

     

    Abstract: By using the peroxidase-antiperoxidase (PAP) immunohistochemical technique, 4 kinds of antisera raised against mammalian hormones were used for immunohistochemical identification and localization of endocrine cells in the gut of grass carp (Ctenopharyngodon idellus), black carp (Mylopharyngodon piceus), common carp (Cyprinus carpio), and culter fish (Erythroculter ilishaeformis). Positive reactions of glucagon-immunoreactive endocrine cells were proved in the whole gut of grass carp (C. idellus). There were only weak positive reactions in the foregut of common carp (C. carpio) and black carp (M. piceus). No positive reactions could be found in the gut of culter fish (E. ilishaeformis). However, only negative reactions were obtained with antisera against pepsinogen, prochymosin and neuron specific erolase in the gut of the 4 cyprinids. In the present paper, the distribution, density and morphological characteristics of glucagon-immunoreactive endocrine cells in different segments of the gut of grass carp (C. piceus) were discribed in detail. The possible physiological functions of the glucagon enter oendocrine cells were discussed and compared with those of glucagon endocrine cells in the gastrointestinal tract of herbivorous mammals.

     

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