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祝璟琳, 李大宇, 邹芝英, 肖炜, 喻杰, 杨弘, 薛良义. 奥尼罗非鱼及其亲本感染无乳链球菌后生理响应变化[J]. 水生生物学报, 2021, 45(6): 1190-1200. DOI: 10.7541/2021.2020.155
引用本文: 祝璟琳, 李大宇, 邹芝英, 肖炜, 喻杰, 杨弘, 薛良义. 奥尼罗非鱼及其亲本感染无乳链球菌后生理响应变化[J]. 水生生物学报, 2021, 45(6): 1190-1200. DOI: 10.7541/2021.2020.155
ZHU Jing-Lin, LI Da-Yu, ZOU Zhi-Ying, XIAO Wei, YU Jie, YANG Hong, XUE Liang-Yi. THE PHYSIOLOGICAL RESPONSE OF HYBRID TILAPIA (OREOCHROMIS NILOTICUS ♀ × OREOCHROMIS AUREUS ♂) AND ITS PARENTS INFECTED WITH STREPTOCOCCUS AGALACTIAE[J]. ACTA HYDROBIOLOGICA SINICA, 2021, 45(6): 1190-1200. DOI: 10.7541/2021.2020.155
Citation: ZHU Jing-Lin, LI Da-Yu, ZOU Zhi-Ying, XIAO Wei, YU Jie, YANG Hong, XUE Liang-Yi. THE PHYSIOLOGICAL RESPONSE OF HYBRID TILAPIA (OREOCHROMIS NILOTICUS ♀ × OREOCHROMIS AUREUS ♂) AND ITS PARENTS INFECTED WITH STREPTOCOCCUS AGALACTIAE[J]. ACTA HYDROBIOLOGICA SINICA, 2021, 45(6): 1190-1200. DOI: 10.7541/2021.2020.155

奥尼罗非鱼及其亲本感染无乳链球菌后生理响应变化

THE PHYSIOLOGICAL RESPONSE OF HYBRID TILAPIA (OREOCHROMIS NILOTICUS ♀ × OREOCHROMIS AUREUS ♂) AND ITS PARENTS INFECTED WITH STREPTOCOCCUS AGALACTIAE

  • 摘要: 研究通过比较杂交子一代奥尼罗非鱼(Oreochromis spp.)与亲本在腹腔注射无乳链球菌(Streptococcus agalactiae)后累积死亡率差异, 血液生理指标、生化指标和脾脏促炎性细胞因子的表达水平变化, 以深入了解奥尼罗非鱼抗病性杂种优势的生理和分子基础。结果显示: 在无乳链球菌人工感染后, 奥尼罗非鱼的累积死亡率显著低于亲本(P<0.05)。感染前奥尼罗非鱼的白细胞数、红细胞数和红细胞压积最高, 感染后3种罗非鱼的白细胞都显著性升高(P<0.05), 红细胞数、红细胞压积和血红蛋白显著性下降(P<0.05), 呼吸暴发受到抑制, 奥尼罗非鱼血液生理指标与父本奥利亚罗非鱼更为接近; 奥尼罗非鱼在感染细菌后的血糖浓度和溶菌酶含量显著高于亲本, 呼吸暴发强于亲本(P<0.05)。定量PCR结果显示感染后3种罗非鱼脾脏TNF-αIL-1βIL-6的mRNA表达水平都显著上升(P<0.05), 3种罗非鱼的TNF-αIL-6都在感染后7h达到峰值, 奥尼罗非鱼IL-1β在感染后48h达到峰值, 尼罗罗非鱼IL-1β在感染后24—48h达到峰值, 奥利亚罗非鱼IL-1β在感染后72h达到峰值; 奥尼罗非鱼的TNF-αIL-6 mRNA表达水平在感染前后都最高, IL-1β mRNA表达水平在感染前和感染后7—48h显著高于亲本(P<0.05)。研究表明杂交育种途径能显著提高鱼体的抗病力, 与亲本相比, 奥尼罗非鱼对无乳链球菌的侵染具有更强的防御能力; 感染后3种罗非鱼促炎性细胞因子都显著升高, 可能参与了宿主对细菌感染的防御, 但奥尼罗非鱼的免疫应答比亲本更活跃, 表现出抗病力杂种优势。

     

    Abstract: Streptococcosis, caused by Streptococcus agalactiae, seriously harms the Chinese tilapia aquaculture industry. Improving the internal disease resistance of tilapia is a preferred method to control this disease. We observed the physiological responses of hybrid tilapia (Oreochromis niloticus♀×O. aureus♂) and their parents after challenged with pathogenic S. agalactiae. The cumulative mortality was recorded at different time points. Blood and spleen samples were collected at 0, 7h, 24h, 48h, 72h, 120h and 168h after infection, respectively. Blood physiological and biochemical indexes were measured between hybrid tilapia and its parents. The expression of pro-inflammatory cytokines in spleen were compared to gain an in-depth understanding of the physiological and molecular basis of the disease resistance heterosis. The result showed that the cumulative mortality of hybrid tilapia was significantly lower than its parents. The white blood cell count, red blood cell count and hematocrit of hybrid tilapia were the highest of three tilapias before infection. The white blood cell count increased significantly after infection, whereas hematocrit and hemoglobin decreased significantly. The infection of fish resulted in a strong inhibition of the respiratory burst, and the blood physiological indexes of hybrid tilapia were closer to those of O. aureus. The result showed that the cumulative mortality of hybrid tilapia was significantly lower than their parents. The white blood cell count, red blood cell count and hematocrit of hybrid tilapia were the highest of three tilapias before infection. The white blood cell count increased significantly after infection, whereas hematocrit and hemoglobin decreased significantly. The infection resulted in a strong inhibition of the respiratory burst, and the blood physiological indexes of hybrid tilapia were closer to those of O. aureus. Hybrid tilapia had higher glucose, lysozyme activity and the respiratory burst compared with their parents. The pro-inflammatory cytokines (TNF-α, IL-1β and IL-6) mRNA levels in the spleen were significantly up-regulated after infection (P<0.05), which peaked at 7h, 48h and 7h, respectively. The TNF-α and IL-6 mRNA levels of hybrid tilapia was the highest of three tilapias before and after infection, and the IL-1β mRNA levels of hybrid tilapia was significantly higher than their parents before infection and at 7−48h after infection. The results showed that hybrid tilapia had stronger defense ability against S. agalactiae infection than their parents with significantly increased pro-inflammatory cytokines after infection. Taken together, this study indicated that the pro-inflammatory cytokines might be involved in host defense against bacterial infection, and the immune response of hybrid tilapia is more active than their parents to support disease resistance.

     

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