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魏炜, 梁成伟, 秦松. 雨生红球藻β-胡萝卜素酮化酶(bkt)启动子功能分析(英文)[J]. 水生生物学报, 2006, 30(6): 747-751.
引用本文: 魏炜, 梁成伟, 秦松. 雨生红球藻β-胡萝卜素酮化酶(bkt)启动子功能分析(英文)[J]. 水生生物学报, 2006, 30(6): 747-751.
WEI Wei, LIANG Cheng-Wei, QIN Song. FUNCTIONAL ANALYSIS OF THE PROMOTER OF BKT ENCODING BETA-CAROTENE KETOLASE IN HAEMATOCOCCUS PLUVIALIS[J]. ACTA HYDROBIOLOGICA SINICA, 2006, 30(6): 747-751.
Citation: WEI Wei, LIANG Cheng-Wei, QIN Song. FUNCTIONAL ANALYSIS OF THE PROMOTER OF BKT ENCODING BETA-CAROTENE KETOLASE IN HAEMATOCOCCUS PLUVIALIS[J]. ACTA HYDROBIOLOGICA SINICA, 2006, 30(6): 747-751.

雨生红球藻β-胡萝卜素酮化酶(bkt)启动子功能分析(英文)

FUNCTIONAL ANALYSIS OF THE PROMOTER OF BKT ENCODING BETA-CAROTENE KETOLASE IN HAEMATOCOCCUS PLUVIALIS

  • 摘要: 单细胞绿藻---雨生红球藻在逆境条件下积累大量的虾青素。β-胡萝卜素酮化酶(bkt)催化在β-胡萝卜素和玉米黄素的β-紫罗酮环C-4位引入酮基的反应,是虾青素合成过程中的关键酶。我们利用凝胶阻滞的方法研究雨生红球藻中bkt基因309bp(-617/-309)启动子区域的转录因子结合位点并发现在-396/-338的59bp探针存在特异的核蛋白结合位点。通过序列分析,发现此59bp区域并不包含TATA或者CAAT-box,而是存在对光、缺氧、p-香豆酸及激素反应的G-box。

     

    Abstract: The unicellular green alga Haematococcus pluvialis accumulates a highvaluable astaxanthin under stress conditions. Betacarotene ketolase (BKT), a key enzyme in astaxanthin biosynthesis in H. p luvialis, catalyzes the conversion of B-carotene to canthaxanthin and zeaxanthin to astaxanthin. Electrophoresis mobility shift assay (EMSA) was used in H. pluvialis to identify transcription factor binding sites within a 309 bp promoter region (-617/-309) of betacarotene ketolase gene and a 59 bp sequence between -396 and -338 bp was found to have a specific binding activity to the nuclear protein. Sequence analysis revealed that this important functional region contains neither TATA nor CAAT box but a Gbox involved in the responsiveness of light, anaerobiosis, pcoumaric acid and hormone.

     

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