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郑济芳, 胡弼, 吴端生. 中华鳖HMG1基因的克隆与序列分析[J]. 水生生物学报, 2006, 30(2): 192-197.
引用本文: 郑济芳, 胡弼, 吴端生. 中华鳖HMG1基因的克隆与序列分析[J]. 水生生物学报, 2006, 30(2): 192-197.
ZHENG Ji-Fang, HU Bi, WU Duan-Sheng. ISOLATION AND SEQUENCE ANALYSIS ON HMG1 GENE FROM THE CHINESE SOFT-SHELL TURTLE(PELODISCUS SINENSIS)[J]. ACTA HYDROBIOLOGICA SINICA, 2006, 30(2): 192-197.
Citation: ZHENG Ji-Fang, HU Bi, WU Duan-Sheng. ISOLATION AND SEQUENCE ANALYSIS ON HMG1 GENE FROM THE CHINESE SOFT-SHELL TURTLE(PELODISCUS SINENSIS)[J]. ACTA HYDROBIOLOGICA SINICA, 2006, 30(2): 192-197.

中华鳖HMG1基因的克隆与序列分析

ISOLATION AND SEQUENCE ANALYSIS ON HMG1 GENE FROM THE CHINESE SOFT-SHELL TURTLE(PELODISCUS SINENSIS)

  • 摘要: 为了解中华鳖(Pelodiscus sinensis)HMG1(High mobility group 1)的基因结构,利用RT-PCR,从中华鳖肝脏组织的总RNA中,克隆并测序了中华鳖HMG1cDNA片段,结果表明,中华鳖HMG1基因的开放读码框(Open reading frame,ORF)长度为606 bp,编码202个氨基酸。中华鳖HMG1多肽链主要包含三个保守的区域:位于多肽链N端的HMG盒区1(第9—80个氨基酸之间);位于多肽链中心的HMG盒区2(第89—162个氨基酸之间);位于多肽链C端的富含酸性氨基酸区域(第163—202个氨基酸之间)。在2个HMG盒区范围内,中华鳖HMG1多肽链与红原鸡、人、虹鳟等物种的HMG1多肽链相比,氨基酸同源性依次为96.5%、74%和67%。排序比较显示,不同物种HMG1多肽链之间的富含酸性氨基酸区域的长度是不同的,暗示了HMG1多肽链富含酸性氨基酸区域的长度可能受到选择压力的影响,但这种选择压力没有使谷氨酸和天冬氨酸这两种酸性氨基酸之间区分开来。系统发生分析表明,脊椎动物HMG1基因的HMG盒区1和盒区2分别形成了2个亚族。本研究首次报道爬行动物的HMG1基因。

     

    Abstract: In order to understand sequence information about turtle HMG1, a cDNA encoding HMG1 protein of the Chinese sof-t shell turtle( Pelodiscus sinensis)was amplified by RT-PCR from liver total RNA, and was cloned, sequenced and analyzed. The results revealed that the open reading frame ( ORF) of turtle HMG1 cDNA is 606 bp long. The ORF encodes 202 amino acid residues. Turtle HMG1 protein contains three conserved regions: two HMG- box domains and one polyacidic region. From the Nterminus, HMG box1 domain(residues 9-80) and HMG box2 domain(residues 89-162) are tandemly arranged.Beginning at position residue 163,the polyacidic region consists of two portions:the first portion(residues 163-181) and the last portion (residues 182-202).The last portion contains a consecutive run of 7 Asp and 14 Glu residues that ends at the C-terminus.Two HMG- box domains of turtleHMG1 protein share higher amino acid identity with homologues sequences of chicken(96.5%) and human(74%) than homologues sequence of rainbow trout( 67%).Length of the polyacidic region from different HMG1 gene is variable,implying that selective forces may play a role in the polyacidic region in HMG1 gene.But,selective forces involved in the evolutionary development of the polyacidic region have not discriminated between Glu and Asp residues.Phylogenetic analysis suggests that HMG box1 and box2 present in vertebrate HMG1 gene form two separate subgroups.To our knowledge,this is the first report of HMG1cDNA sequence in any reptilian.

     

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