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赵俊, 崔淼, 陈湘粦. 异精效应在雌核发育彭泽鲫胚胎发育中的同工酶证据[J]. 水生生物学报, 2004, 28(1): 33-39.
引用本文: 赵俊, 崔淼, 陈湘粦. 异精效应在雌核发育彭泽鲫胚胎发育中的同工酶证据[J]. 水生生物学报, 2004, 28(1): 33-39.
ZHAO Jun, CUI Miao, CHEN Xiang-Lin. ISOZYME EVIDENCES FOR ALLOGYNOGENETIC BIOLOGICAL EFFECTS IN EMBRYOGENESIS OF GYNOGENETIC CRUCIAN CARP(CARASSIUS AURATUS OF PENGZE)[J]. ACTA HYDROBIOLOGICA SINICA, 2004, 28(1): 33-39.
Citation: ZHAO Jun, CUI Miao, CHEN Xiang-Lin. ISOZYME EVIDENCES FOR ALLOGYNOGENETIC BIOLOGICAL EFFECTS IN EMBRYOGENESIS OF GYNOGENETIC CRUCIAN CARP(CARASSIUS AURATUS OF PENGZE)[J]. ACTA HYDROBIOLOGICA SINICA, 2004, 28(1): 33-39.

异精效应在雌核发育彭泽鲫胚胎发育中的同工酶证据

ISOZYME EVIDENCES FOR ALLOGYNOGENETIC BIOLOGICAL EFFECTS IN EMBRYOGENESIS OF GYNOGENETIC CRUCIAN CARP(CARASSIUS AURATUS OF PENGZE)

  • 摘要: 采用聚丙烯酰胺凝胶垂直板电泳技术,对同源和异源精子激发雌核发育彭泽鲫子代胚胎发育过程中四种同工酶(EST,LDH,MDH,ME)的表达情况进行了比较研究.结果表明同源和异源精子激发的胚胎在MDH和LDH同工酶表达上存在明显差异.MDH同工酶的差异主要表现为:在孵出期,异源精子激发的胚胎比同源精子激发的胚胎多出两条谱带MDH4'和MDH5';LDH同工酶的差异表现为:在原肠中期至肌肉收缩期的五个时期中,异源精子激发的胚胎比同源精子激发的胚胎多出4条谱带(LDH9'-12').这种差异说明同源与异源精子对子代胚胎发育过程中同工酶表达的影响不同,可能属于"异精生物学效应"的一种表现形式.

     

    Abstract: Pengze crucian carp, just like silver crucia n carp, is known as gynoge netic crucian carp. It is one of the most popular cultural fishes in China. The offspring of pengze crucian carp activated by heterogeneous sperms show obvious allogynogenetic biological effects in growth, rate of fertilization and other as pects. But like in silver crucian carp, no evidences have been found that the al logynogenetic biological effects exist in morphology or protein phenotype in pen gze crucian carp. That is why there are argument about the phenomenon of allogyn ogenetic biological effects. In this paper, the patterns of expressions in four isozymes (EST, MDH, ME and LDH) were investigated in the embryogenetic stages of gynogenetic crucian carp (Carassius auratus of Pengze) activated by homogen ous and heterogeneous sperms, respectively, used by polyacrylamide gel electroph oresis. The material used here were collected from Nanhai Aquiculture Experiment Base, College of Life Science, South China Normal University. Both MDH and LDH showed apparent differences between the embryos activated by homogenous sperm an d those by heterogeneous sperm. The main differences in MDH exist at hatching st age, which showed two more bands in the isozyme pattern of embryos activated by heterogeneous sperm than those of embryos activated by homogenous sperm. The mai n differences in LDH exist at five stages from middle-gastrula to muscle constr uction, which showed four more bands in the isozyme pattern of embryos activated by heterogeneous sperm than those of embryos activated by homogenous sperm. Mea nwhile, the other two isozymes (EST and ME) detected in this paper did not show apparent differences between the embryos activated by homogenous sperm and those activated by heterogeneous sperm. Furthermore, the speed of development of the embryos activated by heterogeneous sperm is slight faster than that of the embry os activated by homogenous sperm. Although it is difficult to define the reason of the above differences, it can be considered as one form of allogynogenetic bi ological effects.

     

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