鱼肝EROD酶活力诱导作为二噁(口英)的水生态毒理学指标
INDUCTION OF EROD ACTIVITY IN FISH LIVER AS A BIOINDICATOR FOR DIOXIN-LIKE COMPOUNDS IN AQUATIC SYSTEM
-
摘要: 利用离体EROD法对严家湖各氧化塘鱼肝中二噁(口英)的毒性效应进行了定量测定,同时与HRGC/HRMS-MID化学分析结果和野外活体暴露鱼肝中EROD活力诱导结果进行了比较.研究发现,各氧化塘中鱼肝的离体、活体EROD测定结果与化学分析结果之间有着极好的相关性.这不仅揭示了鱼肝细胞色素P450系统以EROD酶活力诱导指示可作为二噁(口英)的水生态毒理学指标的可靠性和准确性,同时也表明了活体EROD和离体EROD生物监测方法两者可以相互补充,用于其他环境生物样品中二噁(口英)类毒物的快速筛选.Abstract: Fish livers from different contaminant ponds of Yan-jia Lake were collected, the TCDD equivalents were measured with EROD bioassay in vitro. The comparative results of EROD bioassay in vitro to chemistry analysis and to EROD bioassay in vivo were summarized. The results from EROD bioassay in vivo and vitro had a good relationship with those from chemical analysis. The studies not only show that the CYP1A1 activity as inducer of EROD is veracious for indicating the toxicity of dioxin-like compounds in aquatic ecosystem, but also reflect that the EROD bioassay in vivo and vitro can be complementarily with each other and useful for a rapid screening of other biosamples in environment.
下载: