Abstract: Crucian carp (Carassius auratus) of Pengze is a bisexual natural gynogenetic. fish. Chromosome preparation was made from leucocyte culture and uncultured kindey cells by injecting PHA and colchicine in vivo. The chromosome number of crucian carp of Pengze was 166 both in male and female. The karyotype is composed of 32m, 40sm, 18st, and 76t arm number (N.F.) is 238. There are obvious differences in chromosome number and karyotype between crucian carp of Pengze and those reported in other natural gynogenetic crucian carp.