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郭超文, 汪鸣, 聂刘旺. 三种龟类动物的细胞遗传研究[J]. 水生生物学报, 1998, 22(1): 17-25.
引用本文: 郭超文, 汪鸣, 聂刘旺. 三种龟类动物的细胞遗传研究[J]. 水生生物学报, 1998, 22(1): 17-25.
Guo Chaowen, Wang Ming, Nie Liuwang. A CYTOGENETIC STUDY ON THREE SPECIES OF TURTLE[J]. ACTA HYDROBIOLOGICA SINICA, 1998, 22(1): 17-25.
Citation: Guo Chaowen, Wang Ming, Nie Liuwang. A CYTOGENETIC STUDY ON THREE SPECIES OF TURTLE[J]. ACTA HYDROBIOLOGICA SINICA, 1998, 22(1): 17-25.

三种龟类动物的细胞遗传研究

A CYTOGENETIC STUDY ON THREE SPECIES OF TURTLE

  • 摘要: 本文以外周血淋巴细胞为材料,首次报道马来闭壳龟和地龟的核型,G带,C带和Ag-NORs,以及平胸龟的G带和C带。发现平胸龟的核型与前人报道的有差异。研究结果表明:平胸龟2=54(14M+4ST+ST+28m),N.F=72,7+6+14。A组No.6qper有一次缢痕.Ag-NOAs位于A组的No.7qter。其全部染色体的着丝粒区均显示阳性C带,并且A组的No.7整条异染色质化;马来闭壳龟Zn=52(18M+4ST+6T+24m),N.E=74,9+5+12。Ag-NORs位于A组No.7qter.全部染色体的着丝粒区均有深染C带,C组的No.1整条异染色质化;地龟核型Zn=52(16M+4ST+6T+26m),N.F=72,8+5+13,A组No.7pper有一次缢痕。Ag-NORs位于A组No.7qper,呈异态。全部染色体着丝粒区C带阳性,A组的No.1qter和No.2,7pqter呈现C带阳性.在三种龟的G带型中,仅马来闭壳龟A组No.8和B组No.2有时呈现同源染色体间异型,其余均可进行同源配对.本文还讨论了核型的演化机制。

     

    Abstract: The conventional karyotypes,G-bands,C-bands and Ag-NORs of three species of turtles have been studied. Platysternon megacephalum Gray has 2n =54(14M+4ST+8T+28m),N. F.=72,7+6+14(Karyotypic formula). The No.6 chromosome of group A has a secondary constriction (SC) in the q per. The Ag-NORs are on the No.7 q ter of group A; Cuora amboinensis (Daudin) has 2n=52(18M+4ST+ 6T+24m),N. F.=74,9+5+12. There are a pair of Ag-NORs in the No.7 q per of group A; Geoemyda spengleri Gmelin has 2n =52(16M+4ST+6T+26m),N.F.=72,8+5+13. The No.7 p per of group A has a SC. The Ag-NORs with hereromorphic phenomenon located on the No.7 q per of group A.All of the centromeric regions of the chromosomes showed staining of C-positihve for these three species. Besides this,the telomeric C-bands was found at the No.1 q ter of group A and the Nos.2,7 p q ter of group C in G. spengleri. The No.7 chromosome of group A of P. megacephalum and the No.1 chromosome of group C of C. amboinensis showed whole staining of C-positive.The chromosomes of G-banded were analyzed. The results show that each homologous pair has its own special G-banded patterns,so is possible to be paired in the karyotype.

     

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