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张宝玉, 李夜光, 耿亚红, 李中奎, 胡鸿钧. 适合大量培养的红球藻藻种的筛选[J]. 水生生物学报, 2004, 28(3): 289-293.
引用本文: 张宝玉, 李夜光, 耿亚红, 李中奎, 胡鸿钧. 适合大量培养的红球藻藻种的筛选[J]. 水生生物学报, 2004, 28(3): 289-293.
ZHANG Bao Yu, LI Ye Guang, GENG Ya Hong, LI Zhong Kui, HU Hong Jun. SELECTION OF HAEMATOCOCCUS STRAINS SUITABLE FOR MASS CULTURE[J]. ACTA HYDROBIOLOGICA SINICA, 2004, 28(3): 289-293.
Citation: ZHANG Bao Yu, LI Ye Guang, GENG Ya Hong, LI Zhong Kui, HU Hong Jun. SELECTION OF HAEMATOCOCCUS STRAINS SUITABLE FOR MASS CULTURE[J]. ACTA HYDROBIOLOGICA SINICA, 2004, 28(3): 289-293.

适合大量培养的红球藻藻种的筛选

SELECTION OF HAEMATOCOCCUS STRAINS SUITABLE FOR MASS CULTURE

  • 摘要: 研究了4个雨生红球藻(Haematococcus pluvialis)品系在10℃、1℃、20℃、2℃和28℃时的生长速率(μ)、生物量、虾青素含量和产量,从中选出适合于大量培养的品系H.pluvialis26和H.pluvialis WZ.2个品系的虾青素含量和产量分别达到2.41%-3.16%,46.1-4.3mg/L和2.40%-2.9%,39.84-40.1mg/L.2个品系的温度适应具有互补性:H.pluvalisWZ适应于较低温度(10℃-20℃),H.pluvialis26适应于较高温度(20℃-28℃),在低温季节使用H.pluvialis WZ,在高温季节使用H.pluvialis 26进行生产,可以延长生产期,提高产量.

     

    Abstract: Haematococcus pluvialis,a kind of unicellular freshwater green algae,has been proved to be one of the most effective natural sources of astaxanthin.Astaxanthin can be used in aquculture,food,medical and cosmetical industries etc,thus it has a promising future,due to its anti oxidation and effective coloration. Aim at the key technological problems for mass culture of the Haematococcus,4 strains of Haematococcus plurialis from our laboratory collection were studied.They were cultured under 10℃,15℃,20℃,25℃,28℃respectively,and were investigated growth rate,biomass,astaxanthin content and productivity.The results are as follow:1):Optimal cultivation temperature of 4 strains were H.pluvalis 26:20 ℃ ;H.pluvalis 30:25 ℃ ;H.pluvalis 34:20 ℃ ;H.pluvalis WZ:15℃respectively.2) Astaxanthin content and productivity of 4 strains were H.pluvalis 26:2.41%—3 16℃ and 46.51—54.35mg/L; H.pluvalis 30:0.94%—1.34% and 7.89—13.13mg/L; H.pluvalis 34:1.41%—1.83% and 10.53 —12 26 mg/L; H.pluvalis WZ: 2.40%—2.59% and 37.84—40.15mg/L.According to culture temperature,astaxanthin content and productivity, H.pluvialis 26 and H.pluvialis WZ were selected as strains suitable for mass culture. The temperature adaptation of H.pluvialis WZ and H.pluvialis 26 are complementary: H.pluvialis WZ is adaptable to lower temperature(10℃—15℃),and H.pluvialis26 is adaptable to higher temperature (20℃—28℃). Using H.pluvialis WZ in cool season and H.pluvialis 26 in warm season respectively for mass culture would extend production duration and thus realize higher productivity.

     

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