留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码
赵凯, 杨公社, 李俊兵, 何舜平. 黄河裸裂尻鱼群体遗传结构和Cyt b序列变异[J]. 水生生物学报, 2006, 30(2): 129-133.
引用本文: 赵凯, 杨公社, 李俊兵, 何舜平. 黄河裸裂尻鱼群体遗传结构和Cyt b序列变异[J]. 水生生物学报, 2006, 30(2): 129-133.
ZHAO Kai, YANG Gong-She, LI Jun-Bing, HE Shun-Ping. PHYLOGENETIC STRUCTURE OF SCHIZOPYGOPSIS PYL ZOVI POPULATIONS FROM MITOCHONDRIAL CYTOCHROME B GENE SEQUENCE VARIATIONS[J]. ACTA HYDROBIOLOGICA SINICA, 2006, 30(2): 129-133.
Citation: ZHAO Kai, YANG Gong-She, LI Jun-Bing, HE Shun-Ping. PHYLOGENETIC STRUCTURE OF SCHIZOPYGOPSIS PYL ZOVI POPULATIONS FROM MITOCHONDRIAL CYTOCHROME B GENE SEQUENCE VARIATIONS[J]. ACTA HYDROBIOLOGICA SINICA, 2006, 30(2): 129-133.

黄河裸裂尻鱼群体遗传结构和Cyt b序列变异

PHYLOGENETIC STRUCTURE OF SCHIZOPYGOPSIS PYL ZOVI POPULATIONS FROM MITOCHONDRIAL CYTOCHROME B GENE SEQUENCE VARIATIONS

  • 摘要: 测定了来自黄河上游和柴达木盆地托索湖的裸裂尻鱼共16个个体的Cytb基因全序列(1141bp),探讨了种群结构和遗传多样性。用MEGA2.1软件分析了碱基组成和序列变异;以青海湖裸鲤、花斑裸鲤和极边扁咽齿鱼为外类群,用PAUP*4.0b10程序构建了单倍型NJ树;用Arlequin Ver.2000程序计算了群体间遗传变异值(Fst)和Nm值以及群体分化概率值。结果显示,来自柴达木水系托索湖的裸裂尻鱼没有形成单系群,Fst=0.204(P0.05),Nm=1.95。初步判断,黄河和柴达木水系托索湖的裸裂尻鱼未显著分化,支持将柴达木裸裂尻鱼(Schizopygopsis kessleri)归并入黄河裸裂尻鱼(Schizopygopsis pylzovi)的形态学结果。两种群核苷酸多样度()分别为0.0012和0.0026,表现为较低水平。根据校正的分子钟推测,黄河和托索湖裸裂尻鱼群体分歧时间为距今7万年左右的更新世末期,结合地理分布的资料和古地质事件,对黄河裸裂尻鱼群体分布水系间的历史联系进行了分析。

     

    Abstract: The complete mtDNA cytochrome b gene sequence (1141bp) was sequenced for 16 Schizopygopsis from the upper reach of the Yellow River and the Tuosuo Lake of the QaidamBasin.The sequence compositions and variations were analyzed using MEGA2.1 software.The Gymnocypris przewalskii and Gymnocypris eckloni and Platypharodon extremus were selected as outgroup,and one molecular phylogenetic tree of 5 haplotypes was constructed using NJ in PAPU 34.0b10 software.The monophyly of the populations of the Schizopygopsis from either the Yellow River or the Tuosuo Lake was not supported.The genetic variability (Fst) and the value of exact test of differentiation between the populations calculated with Arlequin Ver.2000 software were 01204(P0105) and 0.0532 (P0105), respectively.The Nm value deduced from Fst between the populations was 1195.These data suggested that the populations of the Schizopygopsis from the Yellow River and the Tuosuo Lake have not evolved to two different species.Our results support the morphological classification that merging the Schizopygopsis kessleri into Schizopygopsis pylzovi.The nucleotide diversity () exhibited by two populations was low (010012~010026).Inferred from the molecular evolution rate,the divergent time of the S.pylozovi from the Yellow River and the Tuosuo Lake was about 70000 years ago in the late Pleistocene.The faunal connection for population of the in historical was analyzed according to the phylogeography of population distributions and paleo2geographical events.

     

/

返回文章
返回