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刘祥林, 印莉萍, 吴燕川, 高志环, 吴晓强. 满江红鱼腥藻glnA启动区的克隆及在烟草中的活性表达[J]. 水生生物学报, 2000, 24(4): 369-373.
引用本文: 刘祥林, 印莉萍, 吴燕川, 高志环, 吴晓强. 满江红鱼腥藻glnA启动区的克隆及在烟草中的活性表达[J]. 水生生物学报, 2000, 24(4): 369-373.
LIU Xiang-lin, YIN Li-ping, WU Yan-chuan, GAO Zhi-huan, WU Xiao-qiang. THE CLONE OF ANABAENA AZOLLAE GLNA PROMOTER AND ACTIVITY EXPRESSION IN TRANSGENIC TOBACCO[J]. ACTA HYDROBIOLOGICA SINICA, 2000, 24(4): 369-373.
Citation: LIU Xiang-lin, YIN Li-ping, WU Yan-chuan, GAO Zhi-huan, WU Xiao-qiang. THE CLONE OF ANABAENA AZOLLAE GLNA PROMOTER AND ACTIVITY EXPRESSION IN TRANSGENIC TOBACCO[J]. ACTA HYDROBIOLOGICA SINICA, 2000, 24(4): 369-373.

满江红鱼腥藻glnA启动区的克隆及在烟草中的活性表达

THE CLONE OF ANABAENA AZOLLAE GLNA PROMOTER AND ACTIVITY EXPRESSION IN TRANSGENIC TOBACCO

  • 摘要: 利用PCR技术获得满江红鱼腥藻glnA启动区,经克隆测序后构成谷氨酰胺合成酶基因启动子驱动的GUS基因表达载体.用基因枪转化,将表达载体导入烟草中,在其叶片和茎中检测到GUS活性,而在烟草根中未见表达.实验结果对真核生物与原核生物间基因表达调控、转录因子识别的研究以及构建实用载体具有一定价值.

     

    Abstract: The promoter of Anabaena aazollae glnA gene was obtained by PCR procedure.A binary vector containing the chimeric gene of glnA / GUS was constructed after cloning and sequencing.The expression vector was transformed into tobacco by microprojectile bombardment.The GUS activities were examined histochemically in leaves and stems of the transgenic tobacco, but not in its roots.The result was valuable both in the regulation of gene expression, the recognition of transcriptional factors between eukaryota and prokaryota and the construction of practical vectors.

     

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