留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码
姚一甲, 温海深, 吕里康, 王孝杰, 李键爽, 谢颂扬, 左陈鹏, 闫少静, 王志军, 齐鑫. GnIH/GnIHR调控卵胎生许氏平鲉交配行为相关基因表达的研究[J]. 水生生物学报, 2023, 47(10): 1561-1575. DOI: 10.7541/2023.2022.0431
引用本文: 姚一甲, 温海深, 吕里康, 王孝杰, 李键爽, 谢颂扬, 左陈鹏, 闫少静, 王志军, 齐鑫. GnIH/GnIHR调控卵胎生许氏平鲉交配行为相关基因表达的研究[J]. 水生生物学报, 2023, 47(10): 1561-1575. DOI: 10.7541/2023.2022.0431
YAO Yi-Jia, WEN Hai-Shen, LÜ Li-Kang, WANG Xiao-Jie, LI Jian-Shuang, XIE Song-Yang, ZUO Chen-Peng, YAN Shao-Jing, WANG Zhi-Jun, QI Xin. GnIH/GnIHR REGULATES THE EXPRESSION OF GENES RELATED TO MATING BEHAVIOR IN OVOVIVIPAROUS BLACK ROCKFISH (SEBASTES SCHLEGELII)[J]. ACTA HYDROBIOLOGICA SINICA, 2023, 47(10): 1561-1575. DOI: 10.7541/2023.2022.0431
Citation: YAO Yi-Jia, WEN Hai-Shen, LÜ Li-Kang, WANG Xiao-Jie, LI Jian-Shuang, XIE Song-Yang, ZUO Chen-Peng, YAN Shao-Jing, WANG Zhi-Jun, QI Xin. GnIH/GnIHR REGULATES THE EXPRESSION OF GENES RELATED TO MATING BEHAVIOR IN OVOVIVIPAROUS BLACK ROCKFISH (SEBASTES SCHLEGELII)[J]. ACTA HYDROBIOLOGICA SINICA, 2023, 47(10): 1561-1575. DOI: 10.7541/2023.2022.0431

GnIH/GnIHR调控卵胎生许氏平鲉交配行为相关基因表达的研究

GnIH/GnIHR REGULATES THE EXPRESSION OF GENES RELATED TO MATING BEHAVIOR IN OVOVIVIPAROUS BLACK ROCKFISH (SEBASTES SCHLEGELII)

  • 摘要: 为探明许氏平鲉交配诱导机制, 研究以繁殖内分泌及生殖行为重要调控因子促性腺激素抑制激素(GnIH)及其受体为对象, 探究其在卵胎生鱼类中的功能。结果显示, gnih在下丘脑表达量最高, 定位于视前区小细胞体视前核(NPO)、大细胞体视前核(PM)及前室周核(NAPv); gnihr与脑中调控生殖行为的基因kisspeptin1cgnrh共定位; GnIH成熟肽能够结合GnIHR并激活下游CRE信号通路; 脑室注射和腹腔注射结果显示, GnIH成熟肽在生殖轴及血清类固醇激素等水平协同促进性腺成熟并引发交配行为。

     

    Abstract: As an important economic species of cage culture in the Yellow Sea and Bohai Sea, the teleost Sebastes schlegelii has a unique oviviparous reproduction mode. In recent years, due to the decline of wild resources of S. schlegelii, farmers began to experiment with cage-cultured adult fish as parent for artificial reproduction. However, the quantity and quality of offspring produced by cultured parents were significantly lower than those of wild parents. Research showed that this phenomenon was closely related to the number of mating between male and female parents. Gonadotropin-inhibiting hormone (GnIH) was an important regulator of reproductive endocrinology and reproductive behavior. In order to explore the regulatory effect of GnIH on reproductive behavior in S. schlegelii, the open reading frame (ORF) of gnih/gnihr genes were cloned, tissues expression patterns were detected by qPCR, and gnih neurons in the hypothalamus were localized by in situ hybridization. In order to verify the function of GnIH, intraperitoneal and intraventricular injection of synthetic GnIH-Ⅲ were used to detect the changes of the relative expression levels of genes related to mating behavior and reproductive endocrine, respectively. The results showed that the gnih sequence with an ORF of 537 bp, encoding a protein composed of 178 amino acids. The precursor peptide could be processed into 3 mature peptides. The ORF of gnihr gene is 1452 bp, encoding a G protein-coupled receptors with 483 amino acids. The gnih was most highly expressed in the hypothalamus and located in the hypothalamic preoptic area of small cell body preoptic nucleus (NPO), large cell body preoptic nucleus (PM) and anterior periventricular nucleus (NAPv). And gnihr co-localized with kisspeptin1 and cgnrh genes, which regulated reproductive behavior in the brain. GnIH maturation peptide could bind to GnIHR and activate the downstream CRE signaling pathway. The results of ventricular and intraperitoneal injections showed that GnIH maturation peptide promoted gonadal maturation and triggered mating behavior. This study provided a theoretical basis for the mating induction mechanism of S. schlegelii.

     

/

返回文章
返回