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徐旭东, 孔任秋, 刘珂, 王江新, 宁德刚. 集胞藻ORFs侧翼序列的PCR扩增和定向基因插入失活策略[J]. 水生生物学报, 2001, 25(6): 544-550.
引用本文: 徐旭东, 孔任秋, 刘珂, 王江新, 宁德刚. 集胞藻ORFs侧翼序列的PCR扩增和定向基因插入失活策略[J]. 水生生物学报, 2001, 25(6): 544-550.
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XU Xu-dong, KONG Ren-qiu, LIU Ke, WANG Jiang-xin, NING De-gang. PCR AMPLIFICATIONS OF FLANKING SEQUENCES OF ORFs IN THE GENOME OF SYNECHOCYSTIS SP. PCC6803 AND A STRATEGY FOR TARGETED GENE DISRUPTION[J]. ACTA HYDROBIOLOGICA SINICA, 2001, 25(6): 544-550.
Citation: XU Xu-dong, KONG Ren-qiu, LIU Ke, WANG Jiang-xin, NING De-gang. PCR AMPLIFICATIONS OF FLANKING SEQUENCES OF ORFs IN THE GENOME OF SYNECHOCYSTIS SP. PCC6803 AND A STRATEGY FOR TARGETED GENE DISRUPTION[J]. ACTA HYDROBIOLOGICA SINICA, 2001, 25(6): 544-550.
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集胞藻ORFs侧翼序列的PCR扩增和定向基因插入失活策略

PCR AMPLIFICATIONS OF FLANKING SEQUENCES OF ORFs IN THE GENOME OF SYNECHOCYSTIS SP. PCC6803 AND A STRATEGY FOR TARGETED GENE DISRUPTION

    摘要
  • 摘要: 针对集胞藻PCC6803的1927个待定编码基因进行了两侧序列的PCR扩增.4个亚株基因组在.sll0267-sll0268-sll0269区域的PCR扩增产物与KazusaDNA数据存在差异.以叶绿素合成基因chlH和chlL为例,显示三片段连接PCR产物可有效用于集胞藻6803基因组定向插入失活.

     

    Abstract: The flanking sequences of 1927 potential protein-coding genes of Synechocystis sp. PCC6803 were amplified by polymerase chain reactions. The PCR products for the sll0267-sll0268-sll0269 region in the genomes of 4 substrains were inconsistent with those predicted by Kazusa DNA database. As shown with chlorophyll synthesis genes chlH and chlL as the examples, products of 3-piece-ligation PCR can be effectively applied to targeted gene disruptions in the genome of Synechocystis sp. PCC6803.

     

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