PpsbA驱动的类金属硫蛋白基因smtA在鱼腥藻的表达和镉离子抗性的提高

狄利俊, 徐旭东, 孔任秋

狄利俊, 徐旭东, 孔任秋. PpsbA驱动的类金属硫蛋白基因smtA在鱼腥藻的表达和镉离子抗性的提高[J]. 水生生物学报, 2003, 27(5): 551-553.
引用本文: 狄利俊, 徐旭东, 孔任秋. PpsbA驱动的类金属硫蛋白基因smtA在鱼腥藻的表达和镉离子抗性的提高[J]. 水生生物学报, 2003, 27(5): 551-553.
DI Li-Jun, XU Xu-Dong, KONG Ren-Qiu. PpsbA-DRIVEN EXPRESSION OF MT-LIKE PROTEIN GENE smtA IN ANAB-AENA AND INCREASE IN TOLERANCE TO Cd2+[J]. ACTA HYDROBIOLOGICA SINICA, 2003, 27(5): 551-553.
Citation: DI Li-Jun, XU Xu-Dong, KONG Ren-Qiu. PpsbA-DRIVEN EXPRESSION OF MT-LIKE PROTEIN GENE smtA IN ANAB-AENA AND INCREASE IN TOLERANCE TO Cd2+[J]. ACTA HYDROBIOLOGICA SINICA, 2003, 27(5): 551-553.

PpsbA驱动的类金属硫蛋白基因smtA在鱼腥藻的表达和镉离子抗性的提高

基金项目: 

中国科学院回国留学人员择优基金资助

PpsbA-DRIVEN EXPRESSION OF MT-LIKE PROTEIN GENE smtA IN ANAB-AENA AND INCREASE IN TOLERANCE TO Cd2+

  • 摘要: 金属硫蛋白 (Metallothionein,简称MT)是一类富含半胱氨酸、具有很强金属结合能力的小分子量蛋白。
  • [1] Vallee B L. Introduction to metallothionein [J]. Methods Enzymol,1991,205:3-7[2] Olafson R W, Occubbin W D, Kay C M. Primary-and secondary-structure analysis of a unique prokaryotic metallothionein from a Synechococus sp.cyanobacterium [J]. J Biochem.,1988,251:691-699[3] Huckle J W, Morby A P, Turner J S, et al. Isolation of a prokaryotic metallothionein locus and analysis of transcriptional control by trace metal ions [J]. Mol. Microbiol,1993,7:177-187[4] Gupta A, Morby A P, Turner J S, et al. Deletion within the metallothionein locus of Cd-tolerant Synechococus PCC6301 involving a highly iterated palindrome (HIPl) [J]. Mol. Microbiol, 1993,7:189-195[5] Shi J G, Lindsay W Y, Huckle J W, et al. Cyanobacterial metallothionein gene expressed in Escherichia coli: metal-binding properties of the expressed protein [J]. FEBS Letter, 1992, 303:159-163[6] Sambrook J, Fritsh E F, Maniatis T. Molecular Cloning: A Laboratory Manual, 2nd ed. [M]. New York: Cold Spring Harbor Laboratory press, 1989[7] Xu Xudong, Wang Yeqin, Li Shanghao. Construction of biphasic CAT promoter probe vector shuttling between Anabaena (blue-green lagae) and Escherichia coli [J]. Journal of Graduate School, Academia Sinica, 1993,10:203-209.[徐旭东,王业勤,黎尚豪. 鱼腥藻--大肠杆菌启动子CAT探测载体的构建. 中国科学院研究生院学报,1993,10:203-209][8] Elhai J, Wolk C P. Conjugal transfer of DNA to cyanobacteria [J]. Methods Enzymol.,1988,167:747-754[9] Elhai J. Strong and regulated promoters in the cyanobacterium Anabaena PCC 7120[J]. FEMS Microbiol. Lett.,1993,114:179-184[10] Xu X, Kong R, Hu Y. Studies on genetically engineered larvicidalcyanobacterium. [J]. Chinese Journal of Vectorial Biology and Control,1993,4:244-247.[徐旭东,孔任秋,胡玉祥. 基因工程杀蚊幼蓝藻的研究. 中国媒介生物学及控制杂志,1993,4:244-247]

    Vallee B L. Introduction to metallothionein [J]. Methods Enzymol,1991,205:3-7[2] Olafson R W, Occubbin W D, Kay C M. Primary-and secondary-structure analysis of a unique prokaryotic metallothionein from a Synechococus sp.cyanobacterium [J]. J Biochem.,1988,251:691-699[3] Huckle J W, Morby A P, Turner J S, et al. Isolation of a prokaryotic metallothionein locus and analysis of transcriptional control by trace metal ions [J]. Mol. Microbiol,1993,7:177-187[4] Gupta A, Morby A P, Turner J S, et al. Deletion within the metallothionein locus of Cd-tolerant Synechococus PCC6301 involving a highly iterated palindrome (HIPl) [J]. Mol. Microbiol, 1993,7:189-195[5] Shi J G, Lindsay W Y, Huckle J W, et al. Cyanobacterial metallothionein gene expressed in Escherichia coli: metal-binding properties of the expressed protein [J]. FEBS Letter, 1992, 303:159-163[6] Sambrook J, Fritsh E F, Maniatis T. Molecular Cloning: A Laboratory Manual, 2nd ed. [M]. New York: Cold Spring Harbor Laboratory press, 1989[7] Xu Xudong, Wang Yeqin, Li Shanghao. Construction of biphasic CAT promoter probe vector shuttling between Anabaena (blue-green lagae) and Escherichia coli [J]. Journal of Graduate School, Academia Sinica, 1993,10:203-209.[徐旭东,王业勤,黎尚豪. 鱼腥藻--大肠杆菌启动子CAT探测载体的构建. 中国科学院研究生院学报,1993,10:203-209][8] Elhai J, Wolk C P. Conjugal transfer of DNA to cyanobacteria [J]. Methods Enzymol.,1988,167:747-754[9] Elhai J. Strong and regulated promoters in the cyanobacterium Anabaena PCC 7120[J]. FEMS Microbiol. Lett.,1993,114:179-184[10] Xu X, Kong R, Hu Y. Studies on genetically engineered larvicidalcyanobacterium. [J]. Chinese Journal of Vectorial Biology and Control,1993,4:244-247.[徐旭东,孔任秋,胡玉祥. 基因工程杀蚊幼蓝藻的研究. 中国媒介生物学及控制杂志,1993,4:244-247]

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出版历程
  • 收稿日期:  2003-02-16
  • 修回日期:  2003-02-25
  • 发布日期:  2003-09-24

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