一种筛选对虾白斑综合症病毒中和抗体的新方法
A NOVEL METHOD OF SCREENING NEUTRALIZING ANTIBODY AGAINST WHITE SPOT SYNDROME VIRUS USING PRIMARY CULTURED CELLS
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摘要: 用对虾淋巴组织的原代培养细胞与对虾白斑综合症病毒进行相互作用,洗去不能结合的病毒,然后用甲醛固定,再用ELISA法检测所吸附的病毒。当病毒与抗血清保温后,抑制病毒与细胞的吸附能力的抗血清中应含有中和抗体。此方法可以在缺少细胞系的情况下,简便地筛选到中和抗体。
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关键词:
- 细胞原代培养 /
- 对虾白斑综合症病毒(WSSV) /
- 中和抗体
Abstract: According to the characteristics of affinity binding of virus with host cells, a novel method of screening neutralizing antibody against white spot syndrome virus(WSSV)has been established. After interaction of WSSV with primary cultured cells from shrimp lymphoid organ, unbound virus is washed away, and the cells are fixed by formaldehyde. Then the virus, which can bind on the cells, are assayed by ELISA. After incubation of virus with its antiserum, the antiserum that can inhibit virus binding ability to the cells should contain neutralizing antibody. This method has some strong points with handle easy and data stable, which can be used to screen neutralizing antibody under the lack of cell lines. -
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