盐生杜氏藻细胞光合特性的研究
STUDIES ON PHOTOSYNTHETIC PROPERTIES OF DUNALIELLA SALINA CELLS
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摘要: 研究了盐生杜氏藻细胞的光合特性获知：(1)在700-300nm波长范围,出现三个吸收峰,分别位于680,485和440nm处;(2)测定了该藻细胞内ATP／ADP比值,钒酸钠处理可以提高ATP／ADP比值;(3)以培养液为反应介质,测出该藻细胞的光合放氧,钒酸钠抑制放氧速率;(4)用调制式荧光计测出该藻细胞的F0,Fm以及经间隔饱和光脉冲引起的Fm,表明盐生杜氏藻具有正常的光系统结构;(5)该藻细胞可诱导可逆的光状态转换,光系统Ⅱ电子传递抑制剂DCMU和磷酸酯酶抑制剂NaF均阻止光状态Ⅱ向光状态Ⅰ转换;(6)提取了该藻细胞和经氯霉素处理的细胞类囊体膜蛋白制剂,经SDS-PAGE分析表明,两者蛋白带的分布有所差异。Abstract: Photosynthetic propenies of Dunaliella salina cells were inveshgated.The following results were obtained: (1) The absorption spectra of D.salina cells in the range of 700-300nm exhibited three high peaks at 680, 485 and 440nm.(2) The value of ATP / ADP in samples of D.salina cells was measured.The inhibitor of plasma membrane-Sodium vanadate increased the value of ATP/ADP in the cells.(3) Photosynthetic O2-evolution of D.salina cells was measured when the culture fluids were used as reaction medium.lt was found that sodium vanadate delayed the induction, inhibited the rate of photosynthetic O2-evolution.(4) The F0, Fm and Fm of chlorophyll a in D.salina cells were measured by pluse modulation fluorometry.It showed that the abilities of reoxidation of PSⅡ in the cells were normal.(5) Reversible light state transition in the cells were induced by lightdark.DCMU and NaF inhibited the reversion of Cells in state 2 to in state 1.(6) The preparations of thylakoid membranes of control and treatment cells of D.salina by chloromycetin were isolated.Different stan densities of proteins in the control and treatment samples were showed at the pattem of SDS- PAGE.
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[1] 杨雪梅,吴超元.盐藻的研究与现状[J].生命科学,1994,(6):7-10[2] Avron M, Ben-Amotz A. Dunliella: Physiology, Biochemistry, and Biothechnology[M] London: CRC Press,1992[3] Pick U, Karin L, Avron M. Determination of ion content and ion fluxes in the alotolerant alga DunaliellaSalina[J]. Plant Physiology, 1986, 81:92-96[4] 王维光,顾俭本.从叶绿体中提取ATP方法的比较[J].植物生理学通讯,1986,(5):259-265[5] 王国强,肖建平,蔡剑萍等.完整叶绿体的光合作用放氧[J].植物生理学通讯,1980,5:31-36[6] Ebbert V, Godde D. Regulation of thylakoid protein phosphorylation in intact chloroplasts by the activityof kinases and phosphatases[J]. Biochirm. Biophys. Acta, 1994, 187:335-346[7] Schreiber U, Endo T, Mi Hualing, Asada K. Quenching analysis of chlorophyll fluorescence by thesaturation plus method: particular aspects relating to the study of Eukaryotic algae and Cyanobacteria[J].Plant Cell Physiol, 1995, 36:873-882[8] 钟健,彭建新,史晓虹等.鱼腥藻类囊体膜及其性质的研究[J].植物学报,1997,39:359-265[9] 王国强,Selman B R豌豆叶绿体偶联因子腺三磷酶的分离和纯化[J].植物生物学报,1988,14:380-384[10] 徐宜为,免疫检测技术(第二版)[M].北京:科学出版社,1991,21-23[11] 陈根云,缪有刚,李立人.光和蛋白合成抑制剂对水稻RubisCo大、小亚基和RubisCo亚基结合蛋白的基因表达的影响[J].植物生理学报,1993,19:243-249[12] 李德耀,沉允钢,质子动力势各组成部分与光合作用的关系[J].科学通报,1994,39:1712-1715[13] Schreiber U, Rienits K G, ATP-induced photochemical quenching of variable chlorophyll fluorescence[J].FEBS Lett., 1987, 211:99-104[14] 谭新星,许大全,沈允钢,小麦叶片的状态转换涉及PSII向PSI激发能的满溢的变化[J].植物生理学报,1997,23:9-14 杨雪梅,吴超元.盐藻的研究与现状[J].生命科学,1994,(6):7-10[2] Avron M, Ben-Amotz A. Dunliella: Physiology, Biochemistry, and Biothechnology[M] London: CRC Press,1992[3] Pick U, Karin L, Avron M. Determination of ion content and ion fluxes in the alotolerant alga DunaliellaSalina[J]. Plant Physiology, 1986, 81:92-96[4] 王维光,顾俭本.从叶绿体中提取ATP方法的比较[J].植物生理学通讯,1986,(5):259-265[5] 王国强,肖建平,蔡剑萍等.完整叶绿体的光合作用放氧[J].植物生理学通讯,1980,5:31-36[6] Ebbert V, Godde D. Regulation of thylakoid protein phosphorylation in intact chloroplasts by the activityof kinases and phosphatases[J]. Biochirm. Biophys. Acta, 1994, 187:335-346[7] Schreiber U, Endo T, Mi Hualing, Asada K. Quenching analysis of chlorophyll fluorescence by thesaturation plus method: particular aspects relating to the study of Eukaryotic algae and Cyanobacteria[J].Plant Cell Physiol, 1995, 36:873-882[8] 钟健,彭建新,史晓虹等.鱼腥藻类囊体膜及其性质的研究[J].植物学报,1997,39:359-265[9] 王国强,Selman B R豌豆叶绿体偶联因子腺三磷酶的分离和纯化[J].植物生物学报,1988,14:380-384[10] 徐宜为,免疫检测技术(第二版)[M].北京:科学出版社,1991,21-23[11] 陈根云,缪有刚,李立人.光和蛋白合成抑制剂对水稻RubisCo大、小亚基和RubisCo亚基结合蛋白的基因表达的影响[J].植物生理学报,1993,19:243-249[12] 李德耀,沉允钢,质子动力势各组成部分与光合作用的关系[J].科学通报,1994,39:1712-1715[13] Schreiber U, Rienits K G, ATP-induced photochemical quenching of variable chlorophyll fluorescence[J].FEBS Lett., 1987, 211:99-104[14] 谭新星,许大全,沈允钢,小麦叶片的状态转换涉及PSII向PSI激发能的满溢的变化[J].植物生理学报,1997,23:9-14
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