鲤血管内皮细胞的分离培养及初步鉴定
SEPARATION CULTURE AND PRELIMINARY IDENTIFICATION OF THE ENDOTHELIAL CELL OF CYPRINUS CARPIO
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摘要: 鲤动脉球腔内灌注0.1%胶原酶消化,分离到大量内皮细胞及少量成纤细胞,28℃,在加入蛋白含量为:350μg/mL鲤下丘脑粗提液的 1640+20%小牛血清培养液中,细胞生长良好,7d后,接近单层.原代经三次 0.5%柠檬酸胰酶消化逐步淘汰少量成纤维细胞,10d后,得纯内皮细胞,并顺利传至二代,第二代细胞经血凝ⅧR因子酶标检测发现,培养细胞存在血凝ⅧR因子相同抗原,具有内皮细胞的一般特征,从而得到初步鉴定.Abstract: Many endothelial cells and few fibro-cells were separated from the artery ball Cyprinus carpio injected with 0.1% collagense(I). At 28, in the 1640+10% calf bovine serum medium which was supplemented with 350μg/mL carp hypothulumus-derived growth substance, these cells grew well and the cell monolayers were obtained within 7-days period. By three times digestion of 0.5% trypsin-citric acid solution, the pure endothelial cells were gained after 10 days and they were subcultured to which. The second generation were proved to have VIIIR factor antigen by anti-VIIIR factor assay. These cells were identictied with general biological character of endothelial cells.
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Keywords:
- Cyprinus carpio /
- Endothelial cells /
- Culture in vitro /
- Preliminary identification
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