鲢早期发育阶段鱼体脂肪酸组成变化
FATTY ACID COMPOSITION AND THEIR CHANGES DURING EARLY LIFE STAGE OF SILVER CARP HYPOPHTHALMICHTHYS MOLITRIX
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摘要: 用气相色谱仪分析了鲢受精卵和仔鱼的脂肪酸组成及其变化.受精卵中共检测到19种脂肪酸;SFA含量最高,其次为PUFA, MUFA最少.SFA中以1C∶0为主,MUFA中以18C∶1为主,PUFA中以DHA、AA和EPA为主;n-3/n-为2.45.初孵仔鱼中HUFA因卵膜的脱落而减少,以后逐渐积累;鲢仔鱼具有将18C PUFA转化为长链HUFA的能力;在早期发育阶段,鲢仔鱼主要消耗1C∶0作为能量代谢的基质.Abstract: The fatty acid composition and their changes in the fertilized eggs and larvae of silver carp were analyzed by means of gas chromatography(GC). There are 19 fatty acids in the fertilized eggs of silver carp. The proportion of SFA, MUFA and PUFA was almost one third for each group, with SFA being the highest and MUFA being the lowest. 16C∶0 accounts the main part in SFA, 18C∶1 in MUFA, and DHA, AA and EPA in PUFA. The n 3/n 6 ratio in the lipid of the fertilized eggs of silver carp was 2.13. In the newly hatched larvae, many kinds of PUFA were lost accompanying the lost of egg membrane during hatch and DHA was not detected. The larvae of silver carp have the ability of conversion 18C PUFA precursor to HUFA. During early development stage, the larvae of silver carp mainly mobilize 16C∶0 as energy substrates.
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Keywords:
- Silver carp /
- Fertilized eggs /
- Larvae /
- Fatty acid composition
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[1] Cowey CB, Sargent JR. Lipid nutrition in fish[J]. Comp. Biochem. Physiol., 1977,57B:269-273[2] Watanabe T. Lipid nutrition in fish [J]. Comp. Biochem. Physiol., 1982, 73B:3-15[3] Steffens W. Effects of variation in essential fatty acids in fish feeds on nutritive value of freshwater fish for humans [J]. Aquaculture, 1997, 151:97-119[4] 陈少莲,胡传林,华元渝.鲢、鳙肌肉脂肪的脂肪酸组成初步研究[C].鱼类学论文集,1983(第三辑):61-67[5] 刘玉芳.中国5种淡水鱼脂肪酸组成分析[J].水产学报,1991,15:169-171[6] 王学文,张秀杰,杨全珍,等.施用鱼特利Ⅱ型肥对鲢背肌中EPA、DHA含量的影响[J].华中农业大学学报,1997, 增刊:147-152[7] 童圣英.四种鲤科鱼类越冬时脂肪酸组成的变化[J].水产学报,1997,21:373-379[8] Rainuzzo JR, Reitan KI, Olsen Y. The significance of lipids at early stages of marine fish: a review [J]. Aquaculture, 1997, 155:103-115[9] Koven WM, Kissil G Wm, Tandler A Lipid and n-3 requirement of Sparus aurata larvae during starvation and feeding [J]. Aquaculture, 1989, 79: 185-191[10] 熊传喜,谢从新,黎会平,等.建鲤卵和仔鱼发育阶段生化成分的变化[J].华中农业大学学报,1995,14:180[11] Rainuzzo JR, Reitan KI, Jorgensen L et al. Lipid composition in turbot larvae fed live feed cultured by emulsion of different lipid classes [J]. Comp. Biochem. Physiol., 1994, 107A:699-710[12] Fraser A J, Sargent J R, Gamble J C et al. Lipid classes and fatty acid composition as indicators of the nutritional condition of larval Atlantic herring [J]. Am. Fish. Soc. Symp., 1987, 2:129-143[13] Bligh E G, Dyer W J. A rapid method of total lipid extraction and purification. Can. J. Biochem. Physiol., 1959, 37:911-917[14] Coutteau P, Geurden I, Camara M R et al. Review on the dietary effects of phospholipids in fish and crustacean larviculture [J]. Aquaculture, 1997, 155:149-164 Cowey CB, Sargent JR. Lipid nutrition in fish[J]. Comp. Biochem. Physiol., 1977,57B:269-273[2] Watanabe T. Lipid nutrition in fish [J]. Comp. Biochem. Physiol., 1982, 73B:3-15[3] Steffens W. Effects of variation in essential fatty acids in fish feeds on nutritive value of freshwater fish for humans [J]. Aquaculture, 1997, 151:97-119[4] 陈少莲,胡传林,华元渝.鲢、鳙肌肉脂肪的脂肪酸组成初步研究[C].鱼类学论文集,1983(第三辑):61-67[5] 刘玉芳.中国5种淡水鱼脂肪酸组成分析[J].水产学报,1991,15:169-171[6] 王学文,张秀杰,杨全珍,等.施用鱼特利Ⅱ型肥对鲢背肌中EPA、DHA含量的影响[J].华中农业大学学报,1997, 增刊:147-152[7] 童圣英.四种鲤科鱼类越冬时脂肪酸组成的变化[J].水产学报,1997,21:373-379[8] Rainuzzo JR, Reitan KI, Olsen Y. The significance of lipids at early stages of marine fish: a review [J]. Aquaculture, 1997, 155:103-115[9] Koven WM, Kissil G Wm, Tandler A Lipid and n-3 requirement of Sparus aurata larvae during starvation and feeding [J]. Aquaculture, 1989, 79: 185-191[10] 熊传喜,谢从新,黎会平,等.建鲤卵和仔鱼发育阶段生化成分的变化[J].华中农业大学学报,1995,14:180[11] Rainuzzo JR, Reitan KI, Jorgensen L et al. Lipid composition in turbot larvae fed live feed cultured by emulsion of different lipid classes [J]. Comp. Biochem. Physiol., 1994, 107A:699-710[12] Fraser A J, Sargent J R, Gamble J C et al. Lipid classes and fatty acid composition as indicators of the nutritional condition of larval Atlantic herring [J]. Am. Fish. Soc. Symp., 1987, 2:129-143[13] Bligh E G, Dyer W J. A rapid method of total lipid extraction and purification. Can. J. Biochem. Physiol., 1959, 37:911-917[14] Coutteau P, Geurden I, Camara M R et al. Review on the dietary effects of phospholipids in fish and crustacean larviculture [J]. Aquaculture, 1997, 155:149-164
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