Cd2+胁迫对石菖蒲生理生化特性的影响
EFFECTS OF ACORUS TATARINOWII ON PHYBIOCHEMICAL CHARACTERISTICS UNDER Cd~(2+) STRESS
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摘要: 通过水培实验,研究了不同浓度(0、50、200、400μmol/L)下和不同时间内(3、7、11d)Cd2+对石菖蒲(Acorus ta-tarinowiiSchott)叶片抗氧酶活性、叶绿素含量、脂质过氧化程度以及脯氨酸含量的影响。结果表明:(1)超氧化物歧化酶(SOD)活性在3d时随着胁迫浓度的增大而增加,但7d和11d时都是在50μmol/L浓度下达到峰值,然后降低,但都高于对照;除400μmol/L处理下SOD随胁迫时间延长呈先降后升的趋势外,其余浓度处理下均升高。(2)过氧化物酶(POD)活性在胁迫3d和7d时均随着胁迫浓度的增大而增加,11d时在200μmol/L处理下达到峰值,然后降低;各浓度处理组均随着胁迫时间的延长POD活性呈下降趋势。(3)过氧化氢酶(CAT)活性在不同时间内随着胁迫浓度的增加呈先增后降趋势,并且各浓度处理组的CAT均随胁迫时间的延长而增加。(4)叶绿素含量在不同胁迫时间内均在200μmol/L处理下达到峰值,然后降低;除400μmol/L处理下的叶绿素随胁迫时间延长而增加外,其余浓度处理组均呈下降趋势。(5)丙二醛(MDA)含量和脯氨酸(Pro)含量均随着胁迫浓度的增加和胁迫时间的延长而增加。研究结果分析表明石菖蒲对Cd2+胁迫有一定的耐受性。Abstract: The objective of this study was to investigate the Cd2+ stress on the changes of phybiochemical characteristics in Acorus tatarinowii,which would be helpful both to the research of biological function of Cd2+and the biotransformation of Cd2+ by A.tatarinowii.Different concentrations of Cd2+were added to A.tatarinowii and measured phybiochemical characteristics on the 3d,7d and 11d respectively.The effects of different Cd2+ concentrations stress and different stress times on antioxidant activities,chlorophyll contents,membrane lipid peroxidation and Pro content in the leaves of A.tatarinowii were investigated by water culture experiments.The results showed that:(1) The activity of SOD increased with the increasing of Cd2+concentration on 3d,which was up to the peak at 50 μmol/L of Cd2+concentration and then decreased on 7d and 11d,but it was higher than the control.Except it increased first and then decreased with the prolongation of Cd2+stress time under 400 μmol/L,other concentration treatments increased.(2) The activity of POD increased with the increasing of Cd2+concentration on 3d and 7d,which was up to the peak at -00 μmol/L of Cd2+concentration and then decreased on 11d.With the prolongation of Cd2+stress time,POD activity decreased in all concentration treatments.(3) The activity of CAT increased first and then decreased with the increasing of Cd2+concentration in different stress times and increased with the prolongation of Cd2+stress time in all concentration treatments.(4) Chlorophyll content was up to the peak at 00 μmol/L of Cd2+concentration and then decreased in different stress times.Except it increased with the prolongation of Cd2+stress time under 400 μmol/L,other concentration treatments decreased.(5) MDA content and Pro content increased with the increasing of Cd2+concentration and the prolongation of Cd2+stress time.The analysis results showed that Acorus tatarinowii had a certain tolerance to Cd2+.
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Keywords:
- Cd2+stress /
- Acorus tatarinowii /
- Antioxidant /
- Chlorophyll /
- Malondialdehyde /
- Proline
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[1] DiaoW P, NiW Z, NiTH, etal.Heavymetalpollutioninwaterbody:cologicaleffectsandcontrolcountermeasure[J].uangdongTraceElementsScience, 2003, 10(3):1- 5 [刁维, 倪吾钟, 倪天华, 等.水体重金属污染的生态效应与防对策.广东微量元素科学, 2003, 10(3):1- 5] DiaoW P, NiW Z, NiTH, etal.Heavymetalpollutioninwaterbody:cologicaleffectsandcontrolcountermeasure[J].uangdongTraceElementsScience, 2003, 10(3):1- 5 [刁维, 倪吾钟, 倪天华, 等.水体重金属污染的生态效应与防对策.广东微量元素科学, 2003, 10(3):1- 5]
[2] ZhangY, GaoJH.EffectsofCd2+ stressontheseedgerminationandsomephysiologicalcharacteristicsaboutseedlingsofTrifoliumpratens[].ActaAgriculturaeBoreali-occidentalisSinica,007, 16(3):57- 59 [张颖, 高景慧.镉胁迫对红三叶种子发及幼苗生理特性的影响.西北农业学报, 2007, 16(3):7- 59] ZhangY, GaoJH.EffectsofCd2+ stressontheseedgerminationandsomephysiologicalcharacteristicsaboutseedlingsofTrifoliumpratens[].ActaAgriculturaeBoreali-occidentalisSinica,007, 16(3):57- 59 [张颖, 高景慧.镉胁迫对红三叶种子发及幼苗生理特性的影响.西北农业学报, 2007, 16(3):7- 59]
[3] ChengSP, RenF, WolfgangG, etal.Effectsofcadmiumonhlorophyllcontent, hotochemicalefficiency, andphotosyntheticntensityofCannaindicaLinn[J].InternationalJournalofPhytoremediation,002, 4(3):239- 246
[4] ZhouSB, WangCJ, YangHJ, etal.StressresponsesandbioaccumulationofheavymetalsbyZizanialatifoliaandAcoruscalamus[].ActaEcologicaSnica, 2007, 27(1):281- 287 [周标, 王春景, 杨海军, 等.菰和菖蒲对重金属的胁迫反应其富集能力.生态学报, 2007, 27(1):281- 287] ZhouSB, WangCJ, YangHJ, etal.StressresponsesandbioaccumulationofheavymetalsbyZizanialatifoliaandAcoruscalamus[].ActaEcologicaSnica, 2007, 27(1):281- 287 [周标, 王春景, 杨海军, 等.菰和菖蒲对重金属的胁迫反应其富集能力.生态学报, 2007, 27(1):281- 287]
[5] HuaLP, HuaL, WangX D, etal.StudyoneffectofreedoneavymetalpollutioninsedimentsofBaiyangdian[J].JournalfSoilandWaterConservation, 2006, 20(2):102- 105 [滑丽, 华珞, 王学东, 等.芦苇对白洋淀底泥重金属污染程度影响效应研究.水土保持学报, 2006, 20(2):102- 105] HuaLP, HuaL, WangX D, etal.StudyoneffectofreedoneavymetalpollutioninsedimentsofBaiyangdian[J].JournalfSoilandWaterConservation, 2006, 20(2):102- 105 [滑丽, 华珞, 王学东, 等.芦苇对白洋淀底泥重金属污染程度影响效应研究.水土保持学报, 2006, 20(2):102- 105]
[6] LiM Y, ChenH M.AntidepressanteffectofwaterdecoctionofhizomaAcoriTatarinowiiinthebehaviouraldespairanimalmodelsofdepression[].JournalofChineseMedicinalMaterials,001, 24(1):40- 41 [李明亚, 陈红梅.石菖蒲对行为绝望物抑郁模型的抗抑郁作用.中药材, 2001, 24(1):40-1] LiM Y, ChenH M.AntidepressanteffectofwaterdecoctionofhizomaAcoriTatarinowiiinthebehaviouraldespairanimalmodelsofdepression[].JournalofChineseMedicinalMaterials,001, 24(1):40- 41 [李明亚, 陈红梅.石菖蒲对行为绝望物抑郁模型的抗抑郁作用.中药材, 2001, 24(1):40-1]
[7] YangLB, LiSL, HuangYZ, etal.EffectofAcorusgramineusnditsactivecomponent-asaroneonapoptosisofhippocampaleuronsinepilepticyoungrats[J].ChineseTraditionalanderbalDrugs, 2006, 37(8):1196- 1199 [杨立彬, 李树蕾,艳智, 等.石菖蒲及其有效成分a-细辛醚对癫痫幼鼠脑海神经元凋亡的影响.中草药, 2006, 37 (8): 1196-199] YangLB, LiSL, HuangYZ, etal.EffectofAcorusgramineusnditsactivecomponent-asaroneonapoptosisofhippocampaleuronsinepilepticyoungrats[J].ChineseTraditionalanderbalDrugs, 2006, 37(8):1196- 1199 [杨立彬, 李树蕾,艳智, 等.石菖蒲及其有效成分a-细辛醚对癫痫幼鼠脑海神经元凋亡的影响.中草药, 2006, 37 (8): 1196-199]
[8] YuanDH, GaoSX, RenQJ, etal.Studyonpurifiedefficiencyofphosphorusandnitrogenfromomesticsewagebyseveralacrophytesinverticalflowconstructedwetlands[J].JournalofoilandWaterConservation, 2004, 18(4):77- 80 [袁东海,士祥, 任全进, 等.几种挺水植物净化生活污水总氮和总效果的研究.水土保持学报, 2004, 18(4):77- 80] YuanDH, GaoSX, RenQJ, etal.Studyonpurifiedefficiencyofphosphorusandnitrogenfromomesticsewagebyseveralacrophytesinverticalflowconstructedwetlands[J].JournalofoilandWaterConservation, 2004, 18(4):77- 80 [袁东海,士祥, 任全进, 等.几种挺水植物净化生活污水总氮和总效果的研究.水土保持学报, 2004, 18(4):77- 80]
[9] YeJX, HeCQ, ChenSF.AllelopathiceffectofAcorustatarinowiionalgaegrowth[].ActaPhytoecologicaSinica, 1999, 234):379- 384 [叶居新, 何池全, 陈少风.石菖蒲的克藻效期杨集辉等:Cd2+胁迫对石菖蒲生理生化特性的影响 961.植物生态学报, 1999, 23(4):379- 384] YeJX, HeCQ, ChenSF.AllelopathiceffectofAcorustatarinowiionalgaegrowth[].ActaPhytoecologicaSinica, 1999, 234):379- 384 [叶居新, 何池全, 陈少风.石菖蒲的克藻效期杨集辉等:Cd2+胁迫对石菖蒲生理生化特性的影响 961.植物生态学报, 1999, 23(4):379- 384]
[10] XiaoNG, YuRY, YuanM X, etal.Principlesandmethodsofiochemicalexperiments(2ed edition)[M].Beijing:PekingniversityPress.2005, 349- 350 [萧能庚, 余瑞云, 袁明秀,.生物化学实验原理和方法(第二版).北京:北京大学出社.2005, 349- 350] XiaoNG, YuRY, YuanM X, etal.Principlesandmethodsofiochemicalexperiments(2ed edition)[M].Beijing:PekingniversityPress.2005, 349- 350 [萧能庚, 余瑞云, 袁明秀,.生物化学实验原理和方法(第二版).北京:北京大学出社.2005, 349- 350]
[11] ZhangZL, QuW Q.LaboratoryGuideofPlantPhysiology(3rddition)[M].Beijing:HigherEducationPress.2003, 123-24;67- 69;274- 276;258- 259 [张志良, 瞿伟菁.植物理学实验指导(第三版).北京:高等教育出版社.2003,23- 124;67- 69;274 - 276;258- 259] ZhangZL, QuW Q.LaboratoryGuideofPlantPhysiology(3rddition)[M].Beijing:HigherEducationPress.2003, 123-24;67- 69;274- 276;258- 259 [张志良, 瞿伟菁.植物理学实验指导(第三版).北京:高等教育出版社.2003,23- 124;67- 69;274 - 276;258- 259]
[12] ChenLF, SongYL.XuYG, etal.Comparisonforactivitiesofuperoxied dismutaseandcatalasebetween Scab-resistantandusceptiblewheatvarieties[J].ActaPhytopathologicaSinica,997, 27(3):209- 213 [陈利锋, 宋玉立, 徐雍皋, 等.抗赤霉病小麦品种超氧化物歧化酶和过氧化氢酶的活性比.植物病理学报, 1997, 27(3):209- 213] ChenLF, SongYL.XuYG, etal.Comparisonforactivitiesofuperoxied dismutaseandcatalasebetween Scab-resistantandusceptiblewheatvarieties[J].ActaPhytopathologicaSinica,997, 27(3):209- 213 [陈利锋, 宋玉立, 徐雍皋, 等.抗赤霉病小麦品种超氧化物歧化酶和过氧化氢酶的活性比.植物病理学报, 1997, 27(3):209- 213]
[13] XuQS, ShiGX, WangX, etal.GenerationofactiveoxygenndchangeofantioxidantenzymeactivityinHydrillaVerticllatanderCd, CuandZnstress[J].ActaHydrobiologicaSinica,006, 30(1):107- 112 [徐勤松, 施国新, 王学, 等.镉、铜锌胁迫下黑藻活性氧的产生及抗氧化酶活性的变化研究.生生物学报, 2006, 30(1):107- 112] XuQS, ShiGX, WangX, etal.GenerationofactiveoxygenndchangeofantioxidantenzymeactivityinHydrillaVerticllatanderCd, CuandZnstress[J].ActaHydrobiologicaSinica,006, 30(1):107- 112 [徐勤松, 施国新, 王学, 等.镉、铜锌胁迫下黑藻活性氧的产生及抗氧化酶活性的变化研究.生生物学报, 2006, 30(1):107- 112]
[14] YangJR, HeJQ, ZhangGX, etal.ReactionofsomeenzymeactivitiesincropsofdifferenttolerancetothestressofCd[J].ChinaEnvironmentalScience, 1 99 6, 16 (2 ): 113-17 [杨居荣, 贺建群, 张国祥, 等.不同耐性作物中几种活性对Cd胁迫的反应.中国环境科学, 199 6, 16(2):13- 117] YangJR, HeJQ, ZhangGX, etal.ReactionofsomeenzymeactivitiesincropsofdifferenttolerancetothestressofCd[J].ChinaEnvironmentalScience, 1 99 6, 16 (2 ): 113-17 [杨居荣, 贺建群, 张国祥, 等.不同耐性作物中几种活性对Cd胁迫的反应.中国环境科学, 199 6, 16(2):13- 117]
[15] LinD, ZhuC, SunZX.Alt
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