转人乳铁蛋白基因草鱼抗GCHV的初步研究
RESISTANCE TO GCHV OF hLFc TRANSGENIC GRASS CARP
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摘要: 草鱼出血病是由草鱼出血病病毒(GCHV,Grass carpHaemorrhage Virus)引起的爆发性疾病,在草鱼鱼种饲养阶段危害极大,迄今尚无有效的防治方法.人类乳铁蛋白(hLF,human lactoferrin)是人类非特异免疫系统中的重要成员[1].它不仅有抑制细菌生长乃至杀死各种细菌的功能[2],还能提高机体抵抗病毒的能力[3].转hLF基因烟草的抗病研究已经取得可喜的进展[4].那么,hLF是否具有提高低等脊椎动物鱼类的抗病毒能力,是一个具有理论和应用意义的课题.通过转人类乳铁蛋白cDNA(hLFc)的研究,有可能就这个问题提供某些有价值的资料.
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关键词:
- 草鱼 /
- 精子电脉冲介导转基因法 /
- 人类乳铁蛋白 /
- 出血病 /
- 攻毒实验
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[1] Kawasaki Yoshihiro, Sato, Kaoru, Shinmoto, Hiroshi; et al. Role of Basic Residues of Human Lactoferrin in the Interaction with B Lymphocytes [J]. Bioscience, Biotechnology, and Biochemistry, 2000, 64(2):314-318[2] Groenink J. Cationic amphipathic peptides, derived from bovine and human lactoferrins, with antimicrobial activity against oral pathogens [J]. FEMS Microbiology Letters, 1999, 179(2):217-222[3] Marchetti M, Superti, F, Ammendolia, MG. et al. Inhibition of poliovirus type 1 infection by iron-, manganese- and zinc-saturated lactoferrin[J]. Medical Microbiology and Immunology, 1999, 187(4):199-204[4] Zhang Zhanyuan, Coyne DP, Vidaver, AK, et al. Expression of human lactoferrin cDNA confers resistance to Ralstonia solanacearum in transgenic tobacco plants. Phytopathology, 1998. 88(7):730-734[5] 谢岳峰,刘东,邹钧,等. 泥鳅受精卵的电脉冲基因转移[J]. 水生生物学报,1989,13(4):387-389[6] F. Y. T. Sin, U. K. Mukherjee, L. Walker, et al. The application of gene transfer techniques to marin resource management: recent advances, problems and future direction [J]. Hydrobiologia, 1997, 352:263-278[7] Chen, LT, Lu L, Broxmeyer HE. Effects of purified iron-saturated human lactoferrin on spleen morphology in mice infected with Friend virus complex [J]. American Journal of Pathology, 1987, 126(2):285-292[8] 朱作言,许克圣,谢岳峰,等. 转基因鱼模型的建立[J]. 中国科学,1989,2:147-155 Kawasaki Yoshihiro, Sato, Kaoru, Shinmoto, Hiroshi; et al. Role of Basic Residues of Human Lactoferrin in the Interaction with B Lymphocytes [J]. Bioscience, Biotechnology, and Biochemistry, 2000, 64(2):314-318[2] Groenink J. Cationic amphipathic peptides, derived from bovine and human lactoferrins, with antimicrobial activity against oral pathogens [J]. FEMS Microbiology Letters, 1999, 179(2):217-222[3] Marchetti M, Superti, F, Ammendolia, MG. et al. Inhibition of poliovirus type 1 infection by iron-, manganese- and zinc-saturated lactoferrin[J]. Medical Microbiology and Immunology, 1999, 187(4):199-204[4] Zhang Zhanyuan, Coyne DP, Vidaver, AK, et al. Expression of human lactoferrin cDNA confers resistance to Ralstonia solanacearum in transgenic tobacco plants. Phytopathology, 1998. 88(7):730-734[5] 谢岳峰,刘东,邹钧,等. 泥鳅受精卵的电脉冲基因转移[J]. 水生生物学报,1989,13(4):387-389[6] F. Y. T. Sin, U. K. Mukherjee, L. Walker, et al. The application of gene transfer techniques to marin resource management: recent advances, problems and future direction [J]. Hydrobiologia, 1997, 352:263-278[7] Chen, LT, Lu L, Broxmeyer HE. Effects of purified iron-saturated human lactoferrin on spleen morphology in mice infected with Friend virus complex [J]. American Journal of Pathology, 1987, 126(2):285-292[8] 朱作言,许克圣,谢岳峰,等. 转基因鱼模型的建立[J]. 中国科学,1989,2:147-155
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