海水亚硝酸氧化细菌初始富集过程中硝酸盐的定性检测

郭青; 吴灶和; 钱鸣亮

海水亚硝酸氧化细菌初始富集过程中硝酸盐的定性检测

湛江海洋大学, 广东省教育厅水产经济动物病害控制重点实验室, 湛江 524025
2. 中国科学院南海海洋研究所, 广州 510301

基金项目:

广东省科技厅计划项目(2003C20332)

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出版历程
- 收稿日期: 2004-10-13
- 修回日期: 2005-05-24
- 发布日期: 2005-09-24

A QUALITATIVE DETECTING METHOD FOR NITRATE IN THE INITIAL ENRICHMENT SYSTEM OF MARINE NITRITE- OXIDIZING BACTERIA

Zhanjiang Ocean University, The Guangdong Province’s Key Laboratory of Control Diseases of Aquatic Economic Animals, Zhanjiang 524025;
2. South China Sea Institute of Oceanology, The Chinese Academy of Sciences, Guangzhou 510301

摘要

摘要: 本文对海水亚硝酸氧化细菌初始富集过程中硝酸盐的定性检测方法及其适用范围进行了研究。结果表明, 在NaNO2起始浓度为100mg/L的亚硝酸氧化细菌初始富集培养系统中,1mL培养液中残余的NaNO2,可先用 1．0mol/L盐酸溶液20μL和50g/L氨基磺酸铵溶液10-20μL将其去除,然后再用二苯胺试剂对培养液中经亚硝酸氯化细菌转化来的NaNO3进行定性检测,可检测出的NaNO3浓度下限在20mg/L左右。在NaNO2起始浓度不同的富集培养系统中,去除NaNO2所需盐酸溶液、氨基磺酸铵溶液的量可根据其起始浓度按比例相应增减,但NaNO2的起始浓度不宜超过200mg/L。该方法亦适用于淡水亚硝酸氧化细菌初始富集培养过程中硝酸盐的定性检测。
- 亚硝酸氧化细菌 /
- 硝酸盐 /
- 定性检测
Abstract: In the enrichment system of marine nitrite-oxidizing bacteria with 100mg/L of initial concentration of NaNO2,in order to qualitatively detect the nitrate produced by nitrite-oxidizing bacteria,the residual nitrite must be removed firstly, which can be made successfully by adding 20μL of 1.0 mol/L HCl and 10 - 20μL of 50g/L NH4SO3NH2 to per milliliter of media. Then, the nitrate can be qualitatively detected by using diphenylamine reagent. The minimal concentration of NaNO3 that can be detected is about 20mg/L. In the different enrichment system with different initial concentration of NaNO2, the dose of HCl and NH4SO3NH2 used to remove NaNO2 can be regulated in proportion as the initial concentration of NaNO2, but the maximum of its initial concentration should not surpass 200mg/L. Also, this kind of qualitative detecting method for nitrate can be applied in the initial enrichment system of freshwater nitrite-oxidizing bacteria.
- Nitrite-oxidizing bacteria /
- Nitrate /
- Qualitatively detect

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参考文献(5)

[1]	元.土镶徽生物分析方法手册.北京:农业出版社.1986,115-116]
[1]	Madigan M T, Martinko J M, Parker J, Btock Biology of Microorgenisms[M].Beijing: Science Prees.2001,817-820.[M.T.马迪根.徽生物生物学第八版.北京:科学出版社.2001.817-820]
[2]	Xu G H,Zheng H Y.Manual of Boil microorganism analytical methods[M]. Beijing: Agricultural Press.1986, 115-116[许光辉，郑洪
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[4]	Huang X H, Li C L, Liu C W, et al.Studies on two microalgae improving environment of shrimp pond and stengthening anti-disease ability of Penaeus vannamei[J].Acta Hydrobiol. Sinica，2002,26(4) :342-347[黄翔鹄，李长玲，刘楚吾，等.两种微藻改善虾池环境增强丸纳对虾的抗病力的研究.水生生物学报，2002,26(4):342-347]

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海水亚硝酸氧化细菌初始富集过程中硝酸盐的定性检测

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出版历程

A QUALITATIVE DETECTING METHOD FOR NITRATE IN THE INITIAL ENRICHMENT SYSTEM OF MARINE NITRITE- OXIDIZING BACTERIA

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出版历程

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