肌肽对奥尼罗非鱼生长性能的影响
EFFECTS OF D IETARY CARNO SINE LEVELS ON GROWTH PERFORMANCE OF TILAP IA, OREOCHROMIS NILOTICUS @O. AUREUS
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摘要: 本研究探讨了饲料中肌肽的添加对奥尼罗非鱼生长、血清激素水平及肌肉组织抗氧化能力的影响。选取罗非鱼360尾,随机分为A、B、C、D 4个组,每组3个重复,每个重复30尾。在A、B、C、D组的饲料中分别添加0、50、100、200mg/kg剂量的肌肽。常规饲养管理,试验周期60d。结果发现:在试验期间,B、C、D组鱼体的体重和体长均大于A组,特别是试验结束时,B组鱼体的体重、体重生长率(RSGR)和相对体长生长率(RLGR)均显著大于A组(p0.05)。在试验全期,B、C、D组鱼体血清生长激素(GH)、类胰岛素生长因子-1(IGF-1)和三碘甲状腺原氨酸(T3)水平均高于A组,但差异不显著。在第20天,B、C组鱼体肌肉组织中超氧化物歧化酶(SOD)活力显著高于A组(p0.05),D组过氧化氢酶(CAT)活力显著低于A组(p0.05);第40天,B、C、D组的谷胱甘肽过氧化物酶(GSH-Px)活力均显著高于A组(p0.05)。试验结果表明:在饲料中添加适宜剂量(50mg/kg)的肌肽,能改善鱼体肌肉组织的抗氧化能力,提高血清中相关的激素水平,促进鱼体内物质代谢,对鱼体生长性能产生积极影响。Abstract: The effects of carnos ine on growth performance of tilap ia (Oreochromis niloticus @O1aureus ) were investigated1360 tilapiawere randomly allocated into 4 treatments in triplicate aquariumswith 30 fishes per aquarium1Group A wasfed with basic diets as the control group.D iets of group B, C and D were supplemented w ith 50, 100 and 200mg/kgcarnosine respectively.The expermient lasted for 60 days1The results showed that the body weight and body length of tila p ia in the group supplemented with carnos inewere higher than that in the control group, and at the end of the tria,l thebodyweight, RSGR and RLGR of tilapia in the group of low2dosage carnos ine ( 50mg/kg) were s ign ificantly higher thanthe controlgroup(p 0105).D iets inclus ion of carnosine could mi prove the specific activities ofT2AOC, SOD and GSH 2Px in muscle of tilapia, but had a trend of debasing CAT act ivity1On the 20d, the specific act ivities of SOD in the group of lowdosage carnos ine ( 50mg/kg) and medium dosage carnosine ( 100mg/kg) were s ign ificantly higher than that in the controlgroup (p< 0105) respectively, and the specific activit ies of CAT in the group of h igh2dosage carnos ine ( 200mg/kg) wass ignificantly lower than that in the control group ( p< 0105) 1On the 40d, the specific activities ofGSH Px in all groupssupplemented with carnos inewere significantly higher than that in the control group (p< 0105).The results indicated thatthe diet supplemented with carnos ine of appropriate dosage ( 50mg/kg) could mi prove the ant ioxidation inmuscle, the hormone levels in serum, and growth of tilapia.
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Keywords:
- Carnosine /
- Oreochromis niloticus@O1aureus /
- Growth performance /
- Hormone /
- Antioxidation
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