鲤鲫人工多倍体谱系中同工酶和蛋白的基因表达
GENE EXPRESSION CHARACTERISTICS IN ARTIFICIAL POLYPLOID LINEAGE AMONG HYBRIDS BETWEEN COMMON CARP AND CRUCIAN CARP
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摘要: 通过对红鲤、红鲫、镜鲤、鲤鲫杂种二倍体一代,二代, 鲤鲫杂种三倍体,鲤鲫复合三倍体,鲤鲫杂种四倍体一代,二代的同工酶及蛋白电泳谱型和扫描数据分析表明:在鲤鲫人工多倍体谱系中,亲代的等位基因在杂交子代中共有四种表达模式:(1)两亲本基因在子代中共同表达,即共显表达;(2)父本的基因表达受到部分或完全的抑制,即母本的基因优先得到表达;(3)母本的基因表达受到抑制,父本的基因得到表达;(4)双亲本的基因表达均受到一定程度的抑制或都不表达.其中第一种表达模式是主要的模式.根据以上基因在杂交子代中的表达特点,可用同工酶和蛋白电泳图谱将鲤鲫人工多倍体谱系的各种生物型逐一加以区分.Abstract: Analysis and comparisons of expression patterns of isozymes and proteins in artificial polyploid lineage among hybrids between common carp and crucian carp, including red carp, red crucian carp, mirror carp, diploid hybrid between common carp and crucian carp, gynogenetic diploid hybrid, triploid hybrid, multiple triploid hybrid, allotetraploid and its second generation, have been carried. Results revealed that there were 4 expression modes of parental alleles in their offspring:)1)simultaneous expression of both parental alleles,)2)expression of paternal alleles was inhibited, but maternal alleles were preferentially expressed,)3)expression of maternal alleles was inhibited, but paternal alleles were preferentially expressed,)4)alleles of two parental were inhibited to a certain extent or were not expressed completely. Among these 4 expression models the mode)1)was the principal model. According to these gene expression modes, different biotypes of this lineage including diploid, triploid and tetraploid hybrids could be distinguished from each other and from their original parents easily.
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Keywords:
- Artificial polyploid /
- Lineage /
- Biotype /
- Co expression /
- Mode
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[1] 朱蓝菲、蒋一NFDA9.银鲫种内的遗传标记及其在选种中的应用[J].水生生物学报,1987,11(2):104-112[2] 王祖熊,刘峰.白鲢不同繁殖群体中乳酸脱氢酶(LDH)和酯酶(EST)同工酶个体发生多态性的研究[J].水生生物学报,1985,9(3):285-293[3] 吴清江,叶玉珍.具有天然雌核发育特性的人工复合三倍体鲤鱼[J].自然科学进展,1997,7(3):340-344[4] 叶玉珍,吴清江.人工四倍体鲤鲫的产生[J].自然科学进展,1999,9(7):658-661[5] 朱蓝菲.鱼类同工酶和蛋白质的聚丙烯酰胺梯度凝胶电泳法[J].水生生物学报,1992,16(20):183-185[6] Shaw C R, Prasad R.Starch gel electrophoresis of enzymes-a compila tion of recipes. Biochem. Genet., 1970,4:297-320[7] 吴玉萍、叶玉珍、吴清江.基因组叠加生物型的基因表达特性[J].遗传学报 ,2000,27(2):108-113[8] Danzmann R G Down N E. Isozyme expression in F1 hybrids between carp and goldfish. Biochem. Genet., 1982,20:1-15.[9] Wu Yu-ping, Ye Yu-zhen and Wu Ching-jiang. Studies on contributi ons of genome-specific antigen of different biotypes generated by genome additi on. Developmental Reproductive Biology, 1999,8(1):35-40 朱蓝菲、蒋一NFDA9.银鲫种内的遗传标记及其在选种中的应用[J].水生生物学报,1987,11(2):104-112[2] 王祖熊,刘峰.白鲢不同繁殖群体中乳酸脱氢酶(LDH)和酯酶(EST)同工酶个体发生多态性的研究[J].水生生物学报,1985,9(3):285-293[3] 吴清江,叶玉珍.具有天然雌核发育特性的人工复合三倍体鲤鱼[J].自然科学进展,1997,7(3):340-344[4] 叶玉珍,吴清江.人工四倍体鲤鲫的产生[J].自然科学进展,1999,9(7):658-661[5] 朱蓝菲.鱼类同工酶和蛋白质的聚丙烯酰胺梯度凝胶电泳法[J].水生生物学报,1992,16(20):183-185[6] Shaw C R, Prasad R.Starch gel electrophoresis of enzymes-a compila tion of recipes. Biochem. Genet., 1970,4:297-320[7] 吴玉萍、叶玉珍、吴清江.基因组叠加生物型的基因表达特性[J].遗传学报 ,2000,27(2):108-113[8] Danzmann R G Down N E. Isozyme expression in F1 hybrids between carp and goldfish. Biochem. Genet., 1982,20:1-15.[9] Wu Yu-ping, Ye Yu-zhen and Wu Ching-jiang. Studies on contributi ons of genome-specific antigen of different biotypes generated by genome additi on. Developmental Reproductive Biology, 1999,8(1):35-40
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