黄鳝所谓退化性腺(膀胱)黏膜上皮的透射电镜观察

曾嶒; 陈雪芬; 杨春; 谢从新; 周洁

摘要: 应用透射电子显微镜对黄鳝(Monopterus albus)所谓退化性腺(即膀胱)的黏膜上皮进行了研究。根据细胞形态与超微结构的特征,在黄鳝膀胱上皮中识别出六类细胞:表层细胞、淋巴样细胞、多线粒体细胞、亮细胞、椭圆体丰富细胞和基层细胞,但未发现生殖细胞。这六类细胞均含较丰富的细胞器,其中椭圆体性质尚不明确。上皮细胞间存在着由紧密连接、桥粒以及镶嵌连接组成的连接复合体和一些散在的桥粒。结果还发现,收缩状态的黄鳝膀胱上皮细胞间具明显的空隙、空腔;细胞内存在着数量不等的网孔,它们是长指状突起造成的隧道断面;黄鳝膀胱上皮具有典型的AUM斑、镶嵌连接和表层细胞游离面的短指状突起。此外,作者还论证了黄鳝右侧发达性腺是两条性腺的复合体以及它们与左侧的所谓退化性腺同体存在的问题。研究结果支持了作者曾经提出的有关黄鳝所谓退化性腺是发达膀胱的论点。

关键词:

Abstract: The mucouse epithelium of "degenerate gonad"(the urinary bladder)of Ricefield eel, Monopterus albus, was studied with transmission electron microscope. In the epithelium, six different cell types were recognized on the basis of their cell morphologies and their fine structures, but no any gonad-cells. These six were designated as superficial cells, lymph-like cells, mitochondria-rich cells, light cells, ellipticbody-rich cells, and basal cells. In addition, the rich cell organs were found in the all of these cells. But an elliptic body within the fifth cells was an undistinct cell organ in quality. The epithelial cells of the bladder were connected by a junctional complex consisted of by a tight junction, several desmosomes, and a lot of interdigitating junctions. The other some scattered desmosomes were found among the epithelial cells as well. In addition, the epithelium contained lots of obvious gaps and cavities among the cells in the contracted urinary bladder. And holes with unequal in quantity were found within the all of the six cell types, and the holes were the cross sections of the cell tunnels, which were formed when some long fingershape projectors of the adjacent cells were withdrawn. The urinary bladder of M. albus possessed some characteristic structures, such as AUM plaques, interdigitating junctions and projecters on the superficial cell free surface. It was also demonstrated that a gonad of M. albus on right side of the abdominal cavity was a composite-body of two gonads actually, and the degenerate gonad existed on the left side of the same cavity. The argument of us, in which the degenerate gonad of M. albus was putted forward to be the urinary bladder, was supported further.

Keywords:

[1]

曾嶒. 黄鳝腹腔左侧管囊结构的研究初报[J]. 华中农学院学报,1985,4(4):95[2] 曾嶒.黄鳝的泌尿系统及其功能[J]. 水生生物学报,1987,11(1):1-8[3] 中国水产科学研究院珠江水产研究所等,广东淡水鱼类志[M]. 广州:广东科技出版社,1991,357-359[4] 梅文骧. 黄鳝Monopterus albus性转变的探讨[J]. 浙江水产学院学报,1993,12(1):53-57[5] Peachey L D, Rasmussen, H. Structure of the toads urinary bladder as related to its physiology [J]. J. Biophysical Biochemical Cytology. 1961, 10:529-553[6] Choi J K. The fine structure of the urinary bladder of the toad, Buro marinus [J]. J. Cell Biology, 1963, 16:53-72[7] Jones T C. Mohr U. Hunt R D. Urinary System [M]. Newyork:Springer-Verlag.1986,284-290[8] 孟庆闻. 鱼类比较解剖[M]. 北京:科学出版社. 1987,251-269[9] Chan S T H, Phillips J G. The structure of the gonad during natural sex reversal in Monopterus albus(Pisces: Teleostei)[J]. J. Zool.)Lond.), 1967, 151: 129-141[10] 肖亚梅. 黄鳝繁殖生物学研究[J]. 湖南师范大学自然科学学报,1993,16(4):346-349[11] 张小雪,董元凯. 黄鳝性腺发生与分化的研究[J]. 水利渔业. 1994,(5):53-56[12] 杨安峰,王平. 大鼠的解剖和组织[M]. 北京:科学出版社.1985,89[13] 成令忠. 组织学彩色图鉴[M]. 北京:人民卫生出版社. 2000,10-21[14] 钟慈声. 细胞和组织的超微结构[M]. 北京:人民卫生出版社. 1984,7-159[15] Pak Poy R F K, Bentley P J. Fine structure of the Epithelial Cells of the Toad Urinary Bladder [J]. Exp. Cell Res., 1960, 20: 235-237[16] Teshima K, Tomonaga S, K. Yamamoto Structure of the urinary bladder in the Pacific cod [J]. Japan. J. Ichthyology, 1987, 33(4):394-398[17] 秉志. 鲤鱼组织[M].北京:科学出版社. 1983,29-31[18] Grizzle J M, Rogers W A. Anatomy and histology of the Channel catfish[M]. Alabama. 1976,35-39

期刊类型引用(3)

1.	刚德报，王姮，关文娟，魏仁杰，丁君，常亚青. 中国北方海域4种海胆消化道结构. 广东海洋大学学报. 2022(02): 38-45 . 百度学术
2.	李鹏琛，刘炳正，苏唯一，常帅，金晗，常亚青，孙志惠. 基于全长转录组测序挖掘中间球海胆(Strongylocentrotus intermedius)性腺发育相关基因. 基因组学与应用生物学. 2022(Z2): 2062-2075 . 百度学术
3.	孙志惠，崔洲平，宋坚，常亚青. 棘皮动物性别决定与分化研究进展. 大连海洋大学学报. 2021(04): 679-686 . 百度学术