用蛋白磷酸酶抑制法比色法检测水中微囊藻毒素类物质

基金项目:

国家高技术研究发展规划(2001AA641030)

教育部博士点基金20020335078资助

DETECTION OF MICROCYSTIN CLASS COMPOUNDS IN WATER BY COLORIMETRIC PROTEIN PHOSPHATASES INHIBITION ASSAY

1.
Institute of Occupational and Environmental Health, Zhejiang University, Hangzhou, 310031;
2.
College of Biosystem Engineering and Food Science, Hangzhou, 310029;
3.
Institute of Hydrobiology, The Chinese Academy of Sciences, Wuhan, 430072

摘要: 水体富营养化加剧水华的发生已成为全球性环境问题,水华的普遍发生使微囊藻毒素成为了一种存在广、影响大的天然有毒物,对水体尤其是富营养化水体中微囊藻毒素水平的监测是水环境监测的重要任务。
- 微囊藻毒素 /
- 蛋白磷酸酶 /
- 比色法 /
- 水华
- Microcystin /
- Protein phosphatase /
- Colorimetric method /
- Water bloom

[1]	Xu L., Chen J., Xu J. et al, Use of protein phosphatase inhibition assay for measuring microcystin class compounds in aquatic systen [J]. Acta Scientiae Circumstantiae, 1999, 19(5):536-539.[徐立红,陈加平,许建明,等. 用蛋白磷酸酶抑制法测定水体中的微囊藻毒素类物质,环境科学学报,1999,19(5):536-539] Xu L., Chen J., Xu J. et al, Use of protein phosphatase inhibition assay for measuring microcystin class compounds in aquatic systen [J]. Acta Scientiae Circumstantiae, 1999, 19(5):536-539.[徐立红,陈加平,许建明,等. 用蛋白磷酸酶抑制法测定水体中的微囊藻毒素类物质,环境科学学报,1999,19(5):536-539]
[3]	Heresztyn T. and Nicholson B. C.. Determination of cyanobacterial hepatotoxins directly in water using a protein phosphatase inhibition assay [J]. Water Research, 2001, 35:3049-3056
[5]	Wong B., Lam P. K S, Xu L. et al. A colorimetric assay for screening microcystin class compounds in aquatic systems[J]. Chemosphere, 1999, 38:1113-1122
[7]	Douglas O, Mountfort, Suzuki T. et al, Protein phosphatases inhibition assay adapted for determination of total DSP in contaminated mussels[J]. Tocicon, 2001, 39:383-390