几种鱼类线粒体ATP酶活性的比较研究
A COMPARATIVE STUDY OF ACTIVITIES OF THE MITOCHONDRIA ATPase OF THE LIVER CELLS IN SEVERAL FISHES
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摘要: 本文比较了草鱼(Ctenopharyngodon idellus)瓦氏雅罗鱼(Leucisous waleckii)和鲮鱼(Cirrhinus molitorella)在常、低温驯养时,肝细胞线粒体ATP酶活性;并采用吐温80处理线粒体,观察其对线粒体ATP酶活化能Arrhenius图折点温度的影响,讨论了线粒体ATP酶活性与鱼类低温适应能力的相关性。认为鱼类线粒体ATP酶活化能折点温度在常、低温驯养时的差异程度和鱼的抗寒性能有关;低温驯养时,线粒体ATP酶活化能折点温度的高低和鱼的低温耐受能力有关。Abstract: This paper describes a comparative study of the activities of the mitochondria ATP ase of the liver cells in Ctenopharyngodon idella. Leuciscus waleckii and Cirrhina molitorella, acclimated to low and high temperatures. Observations were made on the effect of Tween-80 treatment of the mitochondria on the transition temperature in the Arrhenius plot for the activation energy of the mitochondria ATPase. Compared with fish acclimated at higher temperatures, fish acclimated at lower temperatures showed lower transition temperatures in the Arrhenius plots for the activation energy of their mitochondria ATPase. Treatment with Tween80 resulted in decreased transition temperatures. It was suggested that the magnitude of reduction in the transition temperature when the fish are acclimated to a lower temperature is correlated with the fish's capacity of cold-resistence. The transition temperature is related to tolerance to low temperature.
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Keywords:
- Mitochondria ATPase /
- Transition temperature /
- Tween-80 /
- Activation energy
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