石鲽细菌性败血感染症及其病原细菌研究
STUDIES ON BACTERIAL SEPTICAEMIC INFECTION AND CHARACTERIZATION OF PATHOGENIC BACTERIA ISOLATED FROM STONE FLOUNDER (KAREIUS BICOLORATUS L.)
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摘要: 2001-2002年,分别对两起养殖的石鲽(Stone flounder,Kareius bicoloratusL.)病害进行了检验,均表现为败血症感染特征。经以20尾病(死)石鲽(每起病例各10尾)做病变组织中细菌检查、细菌分离与鉴定、人工感染试验等的病原学检验,表明两起被检病例均为同种的杀鲑气单胞菌(Aeromonas salmonicida)感染;另在其中1起病例所检10尾石鲽的2尾中,同时检出了呈继发感染的不动杆菌(Acinetobacter)。通过对所分离后做纯培养的60株杀鲑气单胞菌6、株不动杆菌分别进行较系统的表观分类学指征(Phenotypic information)及代表菌株16S rRNA基因序列测定与系统发育学分析,表明其中的杀鲑气单胞菌为一个新亚种,不动杆菌为琼氏不动杆菌(Acinetobacter junii)的一个新形态型;又择代表菌株送中国典型培养物保藏中心(CCTCC)进行了表观性状、DNA中C+Cmol%的测定等的复核鉴定,并将其中的杀鲑气单胞菌新亚种定名为杀鲑气单胞菌杀鲽亚种(Aeromonas salmonicidasubsp.flounderacidasubsp.nov.)、将其中的琼氏不动杆菌新形态型定名为琼氏不动杆菌形态型Ⅰ(Acinetobacter juniimorphovarⅠ)。Abstract: We conducted examinations to two cases occurring farmed Stone flounder(Kareius bicoloratus L.)in 2001 and 2002 respectively, all diseased fishes exhibited traits of septicaemia infection. Preliminary pathogen identification studies based on bacteriological examination of damaged tissues, isolation and identification of pathogenic bacteria and experimental infection, showed that two examined cases were infected by the same Aeromonas salmonicida, in addition, Acinetobacter which was thought successive infection was examined from two in examined ten fishes. Identification of extensive phenotypic informations for60 pure cultures of A. salmonicida and 6 pure cultures of Acinetobacter were conducted, the 16S rRNA gene were sequenced to repre-sentative strains, molecular phylogenetic dendrogram was constructed. The results showed that the examined A. salmonicida was identified a new subspecies, the examined Acinetobacter was identified a newmorphotypeof Acinetobacter junii ; the phenotyicin-formations of this bacteria have been re-checked and identified by China Center for Type Culture Collection (CCTCC), and the mol % G +C ratio of the DNA for representative strains were detected, and nemed Aeromonas salmonicida as Aeromonas salmoni-cida subsp. flounderacida subsp. nov. ; and named A. junii as A. junii morphovar Ⅰ.
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