草鱼、中华鳖IL-2活性影响因素的研究
A STUDY OF INFLUENTIAL FACTORS ON IL-2 ACTIVITY OF GRASS CARP, CTENOPHARYNGODON IDELLUS AND CHINESE SOFT-SHELLED TURTLE, TRIONYX SINENSIS
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摘要: 结果显示:当年草鱼种和中华鳖脾细胞培养上清液中能检测出IL-2活性,而且1龄以上草鱼、中华鳖的IL-2活性高于当年孵化的草鱼和中华鳖.草鱼、中华鳖脾细胞在25℃培养温度条件下,其上清液中IL-2活性最高,35℃次之,15℃最低.因此,草鱼、中华鳖IL-2活性在一定范围内是随着年龄增加而增强和依赖温度的.通过小鼠胸腺细胞增殖和对小鼠L929细胞杀伤率的实验表明37℃比25℃检测温度下所测的IL-2活性高,而中华鳖胸腺细胞增殖实验却显示25℃检测温度下IL-2活性高于37℃.本文讨论了年龄、温度对免疫反应影响的机理.Abstract: Interleukin 2(IL-2)activities were tested in splenocyte culture supernatant of grass carp, Ctenopharyngodon idellus and Chinese soft-shelled turtle, Trionyx sinensis under one age. IL-2 activities of grass carp and Chinese soft-shelled turtle above one age were higher than those under one age. Within 15℃ to 35℃ range,IL-2 activities of splenocyte culture supernatant of grass carp and Chinese soft-shelled turtle were the highest at 25℃,the lowest at 15℃. Thereby,IL-2 activities increased with age and depended on temperature within a certain range. However,effects of assay temperature on IL-2 activity of grass carp and Chinese soft-shelled turtle were complicated. By thymocyte(C57BL/6-8 mice)proliferation and L929 cytotoxicity assay,IL-2 activities of grass carp and Chinese soft-shelled turtle at 37℃ was higher than those at 25℃. But by thymocyte(T.sinensis)proliferation assay,IL-2 activity of Chinese soft-shelled turtle at 25℃ was higher than those at 37℃. The influential mechanism of age and temperature on immune response has been discussed.
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immune system of channel catfish(Ictalurus punctatus).I.Leucocyte distribution and phagocyte function in the anterior kidney at 10 degree C[J].Comp Biochem Physiol,1991,100A(4):907-912[20] 李静,陈昌福.低温季节草鱼离体白细胞吞噬活性的研究[J].水生生物学报,1998,22(增刊);132—137 郭琼林.草鱼、中华鳖脾细胞培养上清液IL-2物质的检测[J].水生生物学报,2001,25(1);21—27[2] Hardie L J,Fletcher T C,Secombes C J.Effect of temperature on macrophage activation and production of macrophage activating factor by rainbow trout(Oncorhynchus mykiss)leucocytes[J].Dev Comp Immunol,1994,18(1):57—66[3] Priebe K,Huber C,Maertlbauer E,Terplan G.Detection of antibodies against larvae of Anisakis simplex in saithe Pollachius virens by ELISA[J].J Vet Med B,1991,38:209—214[4] Ourth D D,Ratts V D,Parker N C.Bactericidal complement activity and concentration of immunoglobulin M,transferrin,and protein at different age of channel catfish[J].J Aquat Anim Health,1991,3(4):274—280[5] 江育林,李燕,于平.草鱼免疫反应的初步研究[J].水生生物学报,1991,15(4):321—326[6] Yang X,Zuo W.(杨先乐,左文功)Ralation between water temperature and immune response of grass carp(Ctenopharyngodon idellus C.et V.)[J].Asian Fish Sci,1997,10(2):.169—177[7] Lovely J E,Cabo C,Griffiths S G,et al.Detection of Renibacterum salmoninarum infection in asymptomatic Atlantic salmon[J].J Aquat Anim Health,1994,6(2):126-132[8] Bly J E,Lawson L A,Abdel-Aziz E S,Clem L W.Channel catfish,Ictaturus punctatus,immunity to Saprolegnia sp.[J].J Appl Aquacult,1993,3(1-2):35-50[9] Hrubec T C,Robertson J L,Smith S A,et al.The effect of temperature and water quality on antibody response to Aeromonas salmonicida in sunshine bass(Morone chrysops×Morone saxatilis)[J].Vet Immunol Immunopathol,1996,50(1-2):157-166[10] Secombes C J,Margaret J,Manning J.Comparative studies on immune system of fishes and amphibian:antigen localization in the carp Cyprinus carpio L[J].J Fish Dis,1980,3:399-412[11] Sidky Y A,Auerbach R.Tissue culture analysis of immunological capacity of snapping turtles [J].J Exp zool,1968,167:187-196[12] Wetherall J D,Turner K.Immune response of the Lizard,Tiliqua rugosa[J].Aust.J Exp Biol Med Sci,1972,50:79-95[13] Collazos M E,Barriga C,Ortega-Rincon E.Seasonal variations in the immune system of the tench,Tinca tinca(Cyprinidae):Proliferative response of lymphocytes induced by mitogens [J].J Comp Physiol,1996,165(7):592-595[14] Bly J E,Clem L W.Temperature and teleost immune functions [J].Fish Shellfish Immunol,1992,2(3):159[15] Le Morvan C,Clerton P,Deschaux P,et al.Effects of environmental temperature on macrophage activities in carp[J].Fish Shellfish Immunol,1997,7(3):209-212[16] Lopez C,Carballal M J,Azevedo C,et al.Differential phagocytic ability of the circulating haemocyte types of the carpet shell clem Ruditapes decussatus(Mollusca:Bivalvia)[J],Dis Aquat Org,1997,30(3):209-215[17] Collazos M E,Ortega E,Barriga C.Effect of temperature on immune system of a cyprind fish(Tinca tinca L).Blood phagocyte function at low temperature[J].Fish Shellfish Immunol,1994,4(3):231-238[18] Collazos M E,Barriga C,Ortega E.Seasonal variations in immune system of the cyprinid Tinca tinca.phagocytic function[J].Comp Immunol Microbiol Infect Dis,1995,18(2):105-113[19] Ainsworth A.J,Chen-Dexiang.,Waterstrat,P.R,et al.Effect of temperature on the immune system of channel catfish(Ictalurus punctatus).I.Leucocyte distribution and phagocyte function in the anterior kidney at 10 degree C[J].Comp Biochem Physiol,1991,100A(4):907-912[20] 李静,陈昌福.低温季节草鱼离体白细胞吞噬活性的研究[J].水生生物学报,1998,22(增刊);132—137
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