Abstract: Mature eggs from carp (Cyprinus carpio L.)and loach (Misgurnus anguillicaudatus Cantor)were inseminated with the same species sperms which had been incubated withrecombinant plasmid DNA containing human GH gene in a solution containing 2.9%g lucose,1% sodium citrate,0.2% sodium bicarbonate,0.03% potassium chloride and 30mg/ml bovine albumin.Individuals of 33.3% carp fry and 37.0% loach fry showed tocarry the foreign gene with 4-150 copies per cell by dot blot hybridization and PCR analysis.The studies indicated that sperm could be a vector to carry and introduce foreign gene intofish eggs in the course of fertilization.