嘉陵江大鳍和瓦氏黄颡鱼血液学指标的研究
THE STUDY ON HAEMATOLOGICAL PARAMETERS OF MYSTUS MACROPTERUS AND PELTEOBAGRUS VACHELLII IN JIALING RIVER
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摘要: 研究了大鳍和瓦氏黄颡鱼血液学指标的季节变化及种间差异，旨在为探讨三峡工程所引起的环境变化，对两种鱼所产生的生理生态学影响提供基础资料。于2002年9月至2003年6月期间，分春、夏、秋和冬4个季节在嘉陵江水土镇以下江段共采集大鳍111尾，瓦氏黄颡鱼124尾。测定了这两种鱼的红细胞数量、血红蛋白含量、平均红细胞血红蛋白含量、血清葡萄糖浓度、血清甘油三酯浓度和血清总蛋白浓度等6项血液学指标。结果表明，两种鱼的多项血液学指标均存在显著的季节间差异，血液指标的变化趋势并不是随水温的升高而升高。繁殖期两种鱼的血清甘油三酯浓度均显著降低。两种鱼的血清葡萄糖和甘油三酯浓度存在种间差异，瓦氏黄颡鱼的血清葡萄糖和甘油三酯浓度均显著高于大鳍。Abstract: In order to document the data as a baseline for future study on environmental impact of the Three Gorges Project on the fishes,seasonal variation in haematological parameters of Mystus macropterus and Pelteobagrus vachelli were investigated as well as the difference between the two species in this study. From September,2002 to June, 2003, 111 Mystus macropterus and 124 Pelteobagrus vachelli were collected in Spring, Summer, Autumn and Winter in the Jialing River between Shuitu Town and Ciqikou. Red blood cells, haemoglobin contents, mean cellular haemoglobin contents, serum glucose concentrations,serum triglyceride concentrations, and serum total protein concentrations in the two species were measured. The results indicated that there were significant differences in each haematological parameter among seasons in either of the two species and these haematological parameters did not increase with water temperature. Serum triglyceride concentration in either of the two species decreased significantly in spawning season, Both serum glucose concentration and serum triglyceride concentration was significantly different between the two species. Serum glucose concentration and serum triglyceride concentration in Pelteobagrus vachelli were significantly higher than those in Mystus macropterus in each season.
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Keywords:
- Mystus macropterus /
- Pelteobagrus vachelli /
- Haematological parameters
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[1] Bahmani M, Oryan S, Pourkazemi M, et al. Effects of ecophysiological stress on cellular immunity system of Persian sturgeon Acipenser persicus [R]. Presented at 14th Iranian congress of physiology and pharmacology. 1999, 16-20 May, Tehran, Iran(in Persian)
[2] Lin G H, Zhang F W, Hong Y J, et al. Haematological study of two-years old silver carp, Hypophthalmichthys Molitrix and bighead carp, Aristichthys Nobilix [J]. Acta Hydrobiologica Sinica, 1998, 22(1): 9-16[林光华,张丰旺,洪一江,等.二龄鲢和鳙血液的比较研究.水生生物学报,1998,22(1): 9-16]
[3] Cogswell A T, Benfey T J, Sutterlin A M. The hematology of diploid and triploid transgenic Atlantic salmon (Salmo salar) [J]. Fish Physiology and Biochemistry, 2001, 24:271-277
[4] Bullis S P. Clinical of pathology of temperate freshwater and estuarine fish [M]. In: Fish Medicine, Stoskpf, 1993:232-239
[5] Adham K G, Hassan I F, Taha N, et al. Impact of hazardous exposure to metals in the Nile and Delta lakes on the catfish, Clarias Lazera [J]. Environmental Monitoring and Assessment, 1999, 54:107-124
[6] Wang D S, Luo Q S. The reproductive biology of Mystus macropterus [J]. Journal of Fisheries of China. 1992, 16(1):50-59[王德寿,罗泉笙.大鳍鳠的繁殖生物学研究.水产学报,1992,16(1):50-59]
[7] Wang J H, Xie X J. The chemical compositions and models for predicting energy density in Pelteobagrus vachelli at different seasons [J]. Acta Ecologica Sinica, 2003, 23(1): 122-129[王军辉,谢小军.瓦氏黄颡鱼不同季节鱼体的化学组成及能量密度预测模型.生态学报,2003,23(1): 122-129]
[8] Bahmani M, Kazemi R, Donskaya P. A comparative study of some hematological features in young reared sturgeons (Acipenser persicus and huso huso) [J].Fish Physiology and Biochemistry, 2001, 24:135-140
[9] Gopal V, Parvathy S, Balasubramanian R, Effect of heavy metals on the blood protein biochemistry of the fish Cyprinus carpio and Its use as a bio-indicator of pollution stress [J].Environmental Monitoring and Assessment, 1997, 48:117-124
[10] Soldatov A A, Parfenova I A. The methemoglobin blood level and stability of circulating erythrocytes of the rockfish Scorpaena porcus to osmotic shock under conditions of experimental hypoxia [J]. Journal of Evolutionary Biochemistry and Physiology, 2001, 37(6): 622-625
[11] Montero D, Izquierdo M S, Tort L, Robaina L, et al. High stocking density produces crowding stress altering some physiological and biochemical parameters in gilthead seabream, Sparus aurata juveniles [J]. Fish Physiology and Biochemistry, 1999, 20:53-60
[12] Handy R D, Depledge M H. Physiological responses: their measurement and use as environmental biomarkers in ecotoxicology [J]. Ecotoxicology, 1999, 8: 329-349
[13] Zhang W M, Zhang L H, Shen W Y, et al.The secretion of growth hormone in starved grass carp, Ctenopharyngodon idellus [J].Acta Hydrobiologica Sinica, 2001, 25(3): 236-240[张为民,张利红,沈文英,等.饥饿状态下草鱼生长激素的分泌.水生生物学报,2001, 25(3): 236-240]
[14] Harvey W A.User's guide for Lsmlmw and Mixmdl PC-2 version[M]. Ohio:Ohio State University.1990
[15] Chan R L, Li Q C, Xu B F, et al. The measurement of clinical normal values of hematology and serum biochemistry and urine in SPF miniature pig [J].Chin.J. Lab. Anim.Sci, 2001,11(3):134-137[詹任列,李权超,徐本法,等.SPF小型猪血液学、血液生化正常参考值、尿常规值测定.中国实验动物学杂志,2001,11(3):134-137]
[16] Rotllant J, Tort L.Cortisol and glucose respones after acute stress by net handling in the sparid red porgy previously subjected to crowding stress [J]. Journal of Fish Biology, 1997, 51:21-28
[17] Ruane N M, Huisman E A, Komen J, Plasma cortisol and metabolite level profiles in two isogenic strains of common carp during confinement [J].Journal of Fish Biology, 2001, 59:1-12
[18] Weil L S, Barry T P, Malison J A. Fast growth in rainbow trout is correlated with a rapid decrease in post-stress cortisol concentrations[J]. Aquaculture, 2001, 193:373-380
[19] Collazos M E, Ortega E, Barriga C, and Rodriguz A B, Seasonal variation in haematological parameters in male and female Tinca tinca[J].Molecular and Cellular Biochemistry, 1998, 183:165-168
[20] Henderson R J and Tocher D R. The lipid composition and biochemistry of freshwater fish [J].Pregress in Lipid Research, 1987, 26:281-347
[21] Xie X J, Long T C, Zhang Y G, Cao Z D. Reproduction investiment in the southern catfish (Silurus meridionalis Chen) [J].Journal of Fish Biology, 1998,52;259-271
[22] Norton E C and Macfarlane B R. Lipid class composition of the viviparous yellowtail rockfish over a reproductive cycle[J]. Journal of Fish Biology, 1999, 54:1287-1299
[23] Lund E D, Sullivan C V, Place A R. Annual cycle of plasma lipids in captive reared striped bass: effects of environment conditions and reproductive cycle [J].Fish Physiology and Biochemistry, 2000, 22:263-275
[24] Duan Z H, Sun J Y. Studies on the reproductive biology of Pelteobagrus vachelli (Richardson)[J].Acta Hydrobiologica Sinica, 1999, 23:610-616[段中华,孙建贻.瓦氏黄颡鱼的繁殖生物学研究.水生生物学报,1999,23:610-616]
[25] Krajnovic'-Ozretic' M, Krajnovic'-Ozretic' B.Detection and evaluation of hepatic intoxication in fish[M].1992, In: Gabrielides G.P.(ed.)
[26] Chang Q, Xiong B X, Long Q L.Bio-chemical analysis of haematological parameters in bred eel (Anguills japonica) [J]. Reservoir Fisheries. 1997, 92:16-18[常青,熊邦喜,龙良启.池养鳗鲡血液生化成分分析.水利渔业,1997, 92:16-18]
[27] Zhang H X, Xie X J. The models for predicting energy density of fish body in Mystus macropterus [J]. Acta Hydrobiologica Sinica,2003,27(6):396-401[张昊星,谢小军.大鳍鳠的能量密度及其预测模型.水生生物学报,2003,27(6):396-401
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