金藻吞噬微囊藻产生的无毒变异株与产毒原始株的比较
COMPARISON BETWEEN THE TOXICMICROCYTIS SPECIES AND NON-TOXIC MICROCYSTIS SPECIES ORIGINATED FROM THE COCULTURE OFMICROCYSTIS AND POTERIOOCHROMOAS SP.
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摘要: 分别从喂食三株原始产毒铜绿微囊藻Microcystis aeruginosa(AC、DS和PCC 7820)的金藻Poterioochromonassp.培养物中获得三株藻,以Nest PCR方法(引物对CC/CG和CH/CI)确定此三株藻均为微囊藻属藻株。HPLC测试结果显示这三株藻均不产生微囊藻毒素。显示Poterioochromonassp.具有将产毒微囊藻转化为无毒微囊藻的能力。比较产毒原始株与无毒变异株的生理特性发现,变异株的类胡萝卜素/叶绿素比值高于原始株;而光反应曲线结果表明,变异株的PSⅡ的量子产率和光合作用活力高于原始产毒株,并且变异株在较低光强下就可达到最大的光合作用活力。显示喂食后产生的变异株比原始株有较高的光合作用效率。变异株的藻蓝蛋白/叶绿素比值则低于原始株,光合作用最适光强低于变异株,并且显示产毒原始株通过增加藻蓝蛋白的相对含量来提高对光照的吸收。变异株具有较高的光合作用效率和藻蓝蛋白含量可能是其能够在微囊藻和金藻混合培养的群体中占优势的原因之一。
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关键词:
- Poterioochromonas sp. /
- 微囊藻 /
- 无毒变异株 /
- 色素含量 /
- 光反应曲线
Abstract: Predation of Poterioochrom onas sp. on preyMicrocystis is norm ally a one way process,.i e.,prey cells wereontinuous ly ingested and gradually disappeared. H owever,it w as not alw ays the sam e. Occasionally,a patch of bluegreenolored cells may re-appear from the bottom of the cu lture flask and gradually proliferated. Based on th is phenomenon,xpermi entswere des igned by using three strains ofMicrocy stis-M. aerug inosa AC,DS and PCC 78-0-as prey organism co-cultured with predator Poterioochrom onas sp.,respect ively. A fter one or twom onths,eventually/transformed0icrocystis populationsw ere obtained separately from each of co-cultured treatment. Nest PCR and 16S rDNA analys isdent ified these transformed cells to beMicrocystis sp.. Furthermore,it was astonish ingly found that these three transformedMcrocystis populations had lost the microcystin-producing ab ility. The result strong ly suggested that P oterioochromonasp. played an mi portant role in transform ing the toxicity ofMicrocy stis during predating process. ransformed,non-toxicMicrocystis had higher value of carote inoids/chlorophyll ratio but lower value of cyanophyc in/hlorophy ll ratio com pared with orig inal toxic strains. L ight response curves indicated that the PS?quantum yield andhotosynthetic activity of these non- toxic stra ins were higher than that of original toxic stra ins,moreover,non-toxic strainsch ieved them axmi um photosynthetic act iv ity at low er light intens ity than that of toxic strains. Th ism ight explain why theon-toxic transformed strains outgrew the toxic wild strains in Microcystis and Poteroochrom onas co-culture. -
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