孔雀鱼胚胎心血管发育的显微观察
OBSERVATION OF GUPPY(POECITIA RETICULATA)EMBRYONIC CARDIOVASCULAR DEVELOPING IN VIVO AND IN VITRO
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摘要: 心血管系统的组织分化研究是近年热点课题之一[1].胚体透明且发育速度快的鱼类胚胎由于具备便于观察的特性[2],正广泛用于脊椎动物造血系统的发生、血细胞生成、及血循环系统分化时序、调控因子等研究[3-5].孔雀鱼(Poecilia reticulata Peters)是卵胎生(Ovoviviparity)鱼类的代表种,其卵子在雌鱼的泄殖腔内受精发育,长成鱼后再出生 ;同时孔雀鱼又是一种小型观赏鱼类,在实验室易于饲养,能不断提供实验用卵和胚胎。因此,在本实验室长期进行鱼类组织细胞培养的基础上[6-11],制备孔雀鱼胚胎组织标本,并对早期胚胎及心血管系统进行了体外培养, 观察和比较了孔雀鱼胚胎心血管系统在体内、外的形成过程。
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Keywords:
- Guppy /
- Cardiovascular /
- Embryo development /
- Tissues culture
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[1] Hines P J. Purnell B A, Marx J. Stem cells branch out [J]. Science, 2000, 287: 1417[2] 桂建芳,分子发育生物学研究的理想模式-斑马鱼[J].生物工程进展, 1995, 15(3): 30-33[3] Brownlie A, Zon L. The zebrafish as a modol system for the study of hematopoiesis, zebrafish mutants point the way to novel genes involved in the generation of vertebrate blood cells [J]. BioScience, 1999, 49(5): 382-392[4] Hong Y, Winkler C, Schartl M. Pluripotency and differentiation of embryonic stem cell lines from the medakafish(Oryzias latipes)[J]. Mechanisms Development 1996, 60: 33-44[5] Zon L, Developmental biology of hematopoiesis [J]. Blood, 1995, 86: 2876-2891[6] Zhang, Q Y, Li, Z Q, Jiang, Y L, et al. Discovery of virus pathogen from soft-shelled turtle Trionyx sinesis. Chinese Science Bulletin.1997,41(20): 1987-1999[7] Zhang Q Y, Li Z. Q. Gui J F Studies on morphogenesis and cellular interactions of Rana grylio virus in an infected fish cell line. Aquaculture 1999,175: 185-197[8] Zhang Q Y, Li Z Q, Gui J F. Isolation of a lethal rhabdovirus from the cultured Chinese sucker Myxocyprinus asiaticus [J]. Diseases of Aquatic Organisms, 2000, 42(1): 1-9[9] 张奇亚、李正秋,桂建芳,孔雀鱼胚心的体外培养[J],科学通报,2001,46(11):930-934[10] 张奇亚、李正秋、袁秀平,草鱼椎骨间质细胞的培养 [J].水生生物学报,2000,24(5):567-569[11] 李正秋、张奇亚,鲢低囊胚期胚胎的培养 [J]. 水利渔业,2000,20(6):11 Hines P J. Purnell B A, Marx J. Stem cells branch out [J]. Science, 2000, 287: 1417[2] 桂建芳,分子发育生物学研究的理想模式-斑马鱼[J].生物工程进展, 1995, 15(3): 30-33[3] Brownlie A, Zon L. The zebrafish as a modol system for the study of hematopoiesis, zebrafish mutants point the way to novel genes involved in the generation of vertebrate blood cells [J]. BioScience, 1999, 49(5): 382-392[4] Hong Y, Winkler C, Schartl M. Pluripotency and differentiation of embryonic stem cell lines from the medakafish(Oryzias latipes)[J]. Mechanisms Development 1996, 60: 33-44[5] Zon L, Developmental biology of hematopoiesis [J]. Blood, 1995, 86: 2876-2891[6] Zhang, Q Y, Li, Z Q, Jiang, Y L, et al. Discovery of virus pathogen from soft-shelled turtle Trionyx sinesis. Chinese Science Bulletin.1997,41(20): 1987-1999[7] Zhang Q Y, Li Z. Q. Gui J F Studies on morphogenesis and cellular interactions of Rana grylio virus in an infected fish cell line. Aquaculture 1999,175: 185-197[8] Zhang Q Y, Li Z Q, Gui J F. Isolation of a lethal rhabdovirus from the cultured Chinese sucker Myxocyprinus asiaticus [J]. Diseases of Aquatic Organisms, 2000, 42(1): 1-9[9] 张奇亚、李正秋,桂建芳,孔雀鱼胚心的体外培养[J],科学通报,2001,46(11):930-934[10] 张奇亚、李正秋、袁秀平,草鱼椎骨间质细胞的培养 [J].水生生物学报,2000,24(5):567-569[11] 李正秋、张奇亚,鲢低囊胚期胚胎的培养 [J]. 水利渔业,2000,20(6):11
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