中华绒螯蟹血细胞的显微、亚显微形态结构及其分类
CLASSIFICATION AND MORPHOLOGICAL OBSERVATIONS OF HAEMOCYTES IN ERIOCHEIR SINENSIS BY LIGHT AND ELECTRON MICROSCOPIES
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摘要: 通过相差显微镜和电镜观察,根据中华绒螯蟹血细胞胞质内有无颗粒以及颗粒大小、染色反应、折光性和形成方式的特点,血细胞分为胞质内无颗粒的无颗粒细胞、胞质内只有具折光性和呈淡红色反应大颗粒的大颗粒细胞、胞质内只有无折光性和呈淡蓝色染色反应小颗粒的小颗粒细胞以及胞质内同时具有大颗粒和小颗粒二种颗粒特性的大小颗粒中间型细胞.小颗粒的形成方式是高尔基体成熟面小泡脱离后直接成为小颗粒,而大颗粒的形成方式是高尔基体成熟面小泡脱离后,数个小泡逐渐聚集成蜂窝状大颗粒,进一步发育成熟为均质大颗粒.实验结果表明:三种有颗粒的细胞是互相独立的,可能分别由无颗粒细胞分化而成.Abstract: By observations of phase contrast light and electron microscopies,according to granular existence and granular size?refraction?staining reaction?forming way,haemocytes were classified into four type cells:agranular cell in whose cytoplasm there was no granular;large granular cell in whose cytoplasm there were refractile and red stained large granulars;small granular cell in whose cytoplasm there were non refractile and blue stained small granulars;large and small granular intermediate cell in whose cytoplasm there were large and small types granulars.The forming way of small granulars was that the small vacuoles which were released from the Golgi organelle directly became the small granulars.The forming way of large granulars was that the small vacuoles which were released from the Golgi organelle first gathered together,then formed reticulate dense bodies,and finally matured into even dense large granulars.The experimental results showed that three types granular cells independently developed,could respectively be differentiated from agranular cells.