两种泥鳅不同核质关系下LDH同工酶基因表达的研究
THE EXPRESSION OF LDH ISOZYMIC GENES IN MISGURNUS ANGUILLICAUDATUS AND PARAMISGURNUS DABRYANUS UNDER DIFFERENT RELATIONS BETWEEN NUCLEUS AND CYTOPLASM
-
摘要: 采用聚丙烯酰胺不连续系统凝胶电泳方法分析了泥鳅和大鳞副泥鳅不同杂交组合不同核质关系下胚胎发育阶段(0-145h)中乳酸脱氢酶(LDH)同工酶的分化表达谱式,LDH同工酶的基因表达随细胞质、细胞核不同及胚胎发育各个时期具有不同的个体发育谱式.以泥鳅卵子为细胞质的Ⅰ、Ⅱ两组,杂交组(Ⅱ)LDH同工酶基因在受精后3min至原肠中期雄核基因参与表达与调控,部分基因位点比本交组(Ⅰ)启动与表达的时间要早,两组的管家酶主要来自细胞质.以大鳞副泥鳅为细胞质的Ⅲ、Ⅳ两组,各酶均以细胞质调控为主,其管家酶与泥鳅有很大不同.并具体分析和讨论了不同核质关系下LDH同工酶基因的表达和调控的时空顺序.Abstract: The expression patterns and regulations of LDH isozymic system are investigated in the early developmental stages [from unfertilized eggs to the one chamber air bladder stage(0-145h)] in different combinations between nucleus and cytoplasm of M. anguillicaudatus and P. debryanus. Isozymes are resolved by polyacrylamide gel electrophoresis and then detected by using specific histochemical staining. The expression patterns and regulations of LDH isozymic systems are different in the different combinations of nucleus and cytoplasm. Group I and II had the same cytoplasm of M. anguillicandatus, and the male nucleus genes of LDH are expressed in hybridization group(II)from 3min of post-fertilization to mid gastrula stage. However their gene Products in inbreeding group(I)are not expressed. Some of the isozymic genes in hybridization group are expressed earlier than in inbreeding group. The house keeping enzymes of group I and II are from cytoplasm. Group III and IV have the same cytoplasm of P. dabryanus, the LDH isozmic genes are expressed and regulated by cytoplasm, their house keeping enzymes are very different from those of M. anguillicaudatus. The temporal and spatial expressions and regulations of LDH genes in different combinations between nucleus and cytoplasm are discussed.
-
-
[1] 赵振山,吴清江.人工诱导大鳞副泥鳅雄核发育二倍体克隆鱼的产生[J].遗产学报,1998,25(5):416—421[2] 梁秋齝,梁坚勇,陈 朝,等.大鳞副泥鳅的胚胎发育及鱼种培养[J].水生生物学报,1988,12(1):27—41[3] 郑文彪,泥鳅胚胎和幼鱼发育的研究[J].水产学报,1985,9(1):37—47[4] 张龙翔,张庭芳,李合媛.生化实验方法和技术[M].北京:高等教育出版社,1981,94—111[5] Shaw C R,Prasad R.Starch gel electgrophoresis of enzymes-a compilation of recipes[J].Biochem.Genet,1970,4:291—320[6] 李 康,李渝成,周 暾.两种泥鳅染色体组型的比较研究[J].动物学研究.1983,4(1):75—80[7] 吴力钊,王祖熊.白鲢发育过程中同工酶基因的表达与调控研究[J].水生生物学报.1997,21(1):49—58[8] 吴力钊,王祖熊.鳙鱼同工酶发育遗传学研究[J].水生生物学报.1992,16(1):8—17[9] Philipp D P.Evolution of pattern of differential gene expression:a comparison of the temporal and spatial patterns of isozyme locus expression in two closely related fish species(Northern largemouth bass.Micropterus salmoides,and smallmouth bass,Micropterus dolomieui)[J].J.Exp.Zool.,1979,210:473—488[10] Pontier P J,Hart N H.Creatine kinase gene expression during the development of Brachydanio [J].J.Exp.Zool.,1979,209:283—296[11] Whitt G S,et al.Allelic expression at enzyme loci in an intertribal hybrid sunfish[J].J.Hered.1973,64:55—61[12] 桂建芳、梁绍昌、朱蓝菲,等.鱼类远缘杂交正反交杂种胚胎发育差异的细胞遗传学分析[J].动物学研究.1993,14(2):171—177[13] 许桂珍.鳙移核鱼LDH、MDH同工酶的研究[J].生物技术,1995,5(4):30—33[14] 叶玉珍,吴清江,陈荣德.草鱼和鲤杂交的细胞学研究—鱼类远缘杂交核质不同步现象[J].水生生物学报.1989,13(3):234—239[15] 王祖熊,张锦霞,靳光琴.鱼类杂交不亲和性的研究[J].水生生物学报,1986,10(2):171—179 赵振山,吴清江.人工诱导大鳞副泥鳅雄核发育二倍体克隆鱼的产生[J].遗产学报,1998,25(5):416—421[2] 梁秋齝,梁坚勇,陈 朝,等.大鳞副泥鳅的胚胎发育及鱼种培养[J].水生生物学报,1988,12(1):27—41[3] 郑文彪,泥鳅胚胎和幼鱼发育的研究[J].水产学报,1985,9(1):37—47[4] 张龙翔,张庭芳,李合媛.生化实验方法和技术[M].北京:高等教育出版社,1981,94—111[5] Shaw C R,Prasad R.Starch gel electgrophoresis of enzymes-a compilation of recipes[J].Biochem.Genet,1970,4:291—320[6] 李 康,李渝成,周 暾.两种泥鳅染色体组型的比较研究[J].动物学研究.1983,4(1):75—80[7] 吴力钊,王祖熊.白鲢发育过程中同工酶基因的表达与调控研究[J].水生生物学报.1997,21(1):49—58[8] 吴力钊,王祖熊.鳙鱼同工酶发育遗传学研究[J].水生生物学报.1992,16(1):8—17[9] Philipp D P.Evolution of pattern of differential gene expression:a comparison of the temporal and spatial patterns of isozyme locus expression in two closely related fish species(Northern largemouth bass.Micropterus salmoides,and smallmouth bass,Micropterus dolomieui)[J].J.Exp.Zool.,1979,210:473—488[10] Pontier P J,Hart N H.Creatine kinase gene expression during the development of Brachydanio [J].J.Exp.Zool.,1979,209:283—296[11] Whitt G S,et al.Allelic expression at enzyme loci in an intertribal hybrid sunfish[J].J.Hered.1973,64:55—61[12] 桂建芳、梁绍昌、朱蓝菲,等.鱼类远缘杂交正反交杂种胚胎发育差异的细胞遗传学分析[J].动物学研究.1993,14(2):171—177[13] 许桂珍.鳙移核鱼LDH、MDH同工酶的研究[J].生物技术,1995,5(4):30—33[14] 叶玉珍,吴清江,陈荣德.草鱼和鲤杂交的细胞学研究—鱼类远缘杂交核质不同步现象[J].水生生物学报.1989,13(3):234—239[15] 王祖熊,张锦霞,靳光琴.鱼类杂交不亲和性的研究[J].水生生物学报,1986,10(2):171—179
计量
- 文章访问数: 764
- HTML全文浏览量: 2
- PDF下载量: 454
下载: