凤眼莲根系分泌物对塔玛亚历山大藻的化感作用(英文)

ALLELOPATHIC EFFECTS OF EICHHORNIA CRASSIPES ROOTS ON ALEXANDRIUM TAMARENSE

  • 摘要: 以塔玛亚历山大藻香港株(Alexandriumtamarense,HKstrain)为材料,研究了凤眼莲(Eichhorniacrassipes)根系分泌物及其丙酮、乙酸乙酯提取物对塔玛亚历山大藻的化感作用(Allelopathic effect)。凤眼莲根粉末的用量为0.1g/L、0.5g/L、1.0g/L、1.5g/L和2.0g/L;丙酮、乙酸乙酯提取物的用量为0.01g/L、0.03g/L、0.05g/L和0.07g/L。由于凤眼莲根系丙酮、乙酸乙酯提取物水溶性比较低,实验中,溶剂提取物置于盖玻片上。培养体系中溶剂提取物的实际浓度通过实验前后盖玻片的差值获得。实验结果表明,凤眼莲根粉末用量大于1.5g/L,对塔玛亚历山大藻抑制率100%,0.5g/L和1.0g/L4d后开始有促进生长的作用。凤眼莲根系丙酮、乙酸乙酯提取物的实际浓度达到0.038g/L、0.022g/L时,对塔玛亚历山大藻抑制超过50%。凤眼莲作为杀藻剂的材料有一定的应用前景。

     

    Abstract: Allelopathic effects of dry powder and extract of acetone/ethyl acetate of Eichhornia crassipes roots on Alexandrium tamarense(HKstrain)were evaluated 0.1 g,0.5g,1.0g,1.5g and 2.0g/L of E1crassipes root powder were added,respectively1Due to low solubilityof extracts,a control of slide was used as carrier to measure the actual concentration of extracts in the cultures.At first,0.01g,0.03g,0.05g,0.07g/L of extractswere put into the weighted slides1Then the slideswere added to the flasks containing 100mL of A1tamarense1At the endof the experiment,the slideswere takenout,dried andweighted1The actual concentrationof extracts in the cultures wasobtainedfrom the loss in weight1The results showed that the growthof A1tamarense was absolutely inhibited when the algae was exposured to dry powder of E1crassipes roots at above 1.5g/L1When the concentration of dry powder of E1crassipes roots were 0.5g/L and 1.0g/L,the growth of algae increased after 3 days1The growth of A1tamarense was also inhibited at the actual concentration of 0.038g/L and 0.022g/L extract of acetone and ethyl acetate of E1crassipes roots,respectively. It suggested that E cras-sipes could be a potential algaecide to control harmful algal blooms

     

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