鲤维生素E缺乏的病理学研究
STUDY ON THE PATHOLOGY OF VITAMIN E DEFICIENCY IN COMMON CARP (CYPRINUS CARPIO)
-
摘要: 本试验分别以0、25、50、100mg/kg饲料的维生素E水平进行了鲤维生素E缺乏的病理学和血液学研究。经20周的饲喂试验,各试验组的发病率和死亡率与日粮中维生素E水平呈负相关,其发病率分别为47.5%、22.50%、10.0%和0%,死亡率分别为22.5%、12.5%、5.0%和0%。病鱼出现特征性的背部两侧肌肉萎缩变薄,呈刀刃状的“瘦背病”、脊柱前凸及以突眼、竖鳞和腹水、体侧红肌纤维退色变白为特征的渗出性素质样病变。组织学上最突出的变化表现为营养性肌病、骨骼肌纤维变性、坏死,残存的肌纤维萎缩变细;心肌纤维变性、坏死;肝脏发生营养性肝病,肝细胞变性、坏死,甚至溶解消失,形成溶解灶。在肝细胞内可见大量黄色或棕黄色、圆形或椭圆形的蜡样质沉着。性腺发育障碍,精巢内精原细胞、精母细胞和精子细胞数量显著减少;卵巢发育迟缓,卵母细胞数量减少。胰腺变性、坏死。肾小球肿大,细胞增生,肾小管上皮细胞变性。超微结构上,骨骼肌横纹模糊不清或消失,肌浆凝固,核膜间隙增宽,核固缩;骨骼肌纤维、心肌纤维、肝细胞和肾小管上皮细胞线粒体肿胀,嵴断裂、溶解、有的呈囊泡化,内质网扩张,脱核糖体颗粒。Abstract: The pathology of vitamin E deficiency in common carp (cyprinus carpio)was studied.Juvenile carp weighing about 60g were fed a purified diet which was supplemented with vitamin E with 0, 25, 50 and 100mg/kg, respectively for 20 weeks.The morbidity and mortality were respectively 47.5%, 22.5%, 10.0%, 0 and 22.5%, 12.5%, 5.0%, 0.The fish fed vitamin E deficient diet occurred “thin back disease”, lordosis and exudative diathesis with exophthalmus, scale erection and ascites.Gross changes showed skeletal muscular dystrophy, especially in both sides of the back.The red muscle in body sides became white muscle ;the liver was swollen with yellow color or blue color in last stage ;the heartwas enlargement with hemorrhage in epicardium ;the kidney was swollen with hyperemia and hemorrhage ;the sexual gland with dysplasia showed that the weight and volume were decreased.Histopathologically, the changes showed nutritional muscular dystrophy, hepatosis dietetica, gonadal dysplasia, degeneration and necrosis of pancreas.The skeletal muscles showed degeneration and necrosis, dystrophy of survival muscle fibers ;the cardiacmuscles showed degeneration and hemorrhage ;the liver showed degeneration, necrosis and many yellow or brown-yellow ceroidlike dense bodies in liver cells;the pancreas showed degeneration and necrosis ;the kidney showed enlargment of glomerulus, degeneration in renal tubular epithelial cells ;the spermary showed the quantities reduce of spermatogonia, primary spermatocyte, secondary spermatocyte and spermatid ;the ovary showed the quantities decreased of follicle.Ultrastructurally, the mitochondria of the cardiacmuscle cells, the liver cells and renal tubular epithelial cells showed swollen with disintegration and lysis of caritas ;the rough endoplasmic reticulum were enlargment with desquamation of ribosome ;the karyotheca of skeletal muscle cells appeared broadening and the nuclears showed distortion with reduced electron density.
-
Keywords:
- Vitamin E deficiency /
- Pathology /
- Cyprinus carpio
-
-
[1] Liu X X.The biology function of the vitamin E [J].The Agricultural Science and Technology Theory Cluster, 1999, 19(2):81-85 [刘湘 新.维生素E 的生物学功能.农业科技论丛, 1999, 19(2):81-85]
[2] Wi se D J.Eff ects of dietary selenium and Vitamin E on red blood cell preoxidation, gluatthione peroxideae activity and macrophage superoxide anion production in channel catfish [J].J.Aquat. Anim. Health, 1993,5:177-182
[3] Zeng H.The fish nourishment demand [M].The Chinese Agriculture Science and Technology Press.1993, 28-29 [曾虹.鱼类营养需 要.中国农业科技出版社.1993, 28-29]
[4] Li A J.The marine products animal nourishment and animal feed learn [M].Peking:Chinese Agri cultural Press.1995, 36-46 [李爱杰. 水产动物营养与饲料学.北京:中国农业出版社.1995, 36-46]
[5] He H, Lawrence A L.Vitamin E requirement of Penaus vannamei [J].Aquac, 1993, 118(34):245-255
[6] Akeuchi T, Watanabe K, Satoh S.Requirement of grass carp fingerlings for alpha-tocopherol [J].Bulletion of the JapaneseSociety of Scientific Fisheries,1992, 58(9):1743-1749
[7] Bassler K H.The problematic nature of vitamin E requirements:net vitamin E [J].Zeitschrift-fur-Ernahrunfswissenschaf t, 1991, 30(3): 174-180
[8] Hilton J N.The interaction of vitamins, minerals and diet composition in the diet of f ish [J].Aquac, 1989, 79(1):223-244
[9] Ji S L, Si F Y.The pathologic study on the chcken vitamin E deficient disease [J].The Pasturage and Veterinary in Hunan, 1997, 3: 14-15 [纪素玲, 四发益.雏鸡E 缺乏症的病理学研究.湖南 畜牧兽医, 1997,3:14-15]
[10] Watanabe T, Takashima F.Requirement of young carp for α-tocopherol [J].Bull.Jpn.Soc.Sci.Fish, 1970, 36:972-976
[11] Watanabe T, Takashima F.Dietary lipid levels and α-tocopherol requirement of carp [J].Bull.Jpn.Soc.Sci.Fish,1981, 47:1585-1590
[12] Moccia-R D, Hung S S O, Slinger S J.Effect of oxidized fish oil, vitamin E and ethoxyquin on the histopathology and haematology of rainbow trout, Salmo gairdneri Rishardson [J].Journal of Fish Disease, 1984, 7:269-282
[13] Bai S C, Lee K, Wi lson R P.Different levels of dietary DL-alphatocopheryl acetate affect the vitamin E status of juvenile Korean rockfish, Sebasters schlegeli.Fish nut rition and feeding, Proceedings of the seventh international symposium on feeding and nut rition in fi sh, college station, Texas, Usa, 11-15 August 1996 [J].Aquaculture, 1998, 161:1-4,405-414
[14] Roem A J, Kohler C C, Stickney R R.Vitamin E requirement s of blue tilapia, oreochromis aureus (steindachner), in relation to dietary lipid level [J].Aquac, 1990, 87(2):155-164
[15] Takeuchi T, Watanabe K, Satoh S.Requirement of grass carp fingerlings for alpha-tocopherol [J].Bulletion of theJapanese Society of Scientific Fisheries.1992,58(9):1743-1749
[16] Chan A C, Hegarty P V J.Morphological changes in skeletal muscles in vitamin E deficient and rabbits [J].Bri tish Journal of Nutritionlovastatin in rets fed a vitamin E deficient diet [J].Biochemical Archives, 1991, 7(2):85-94
[18] Ren Z L, Huo Q G, Zeng H.The Oxidized fish oil to the influence of the produce capability and the muscular tissue structure in Cyprinus carpio [J].J Animal Nourishment, 2001, 13(1):59-64 [任泽林, 霍启光, 曾虹.氧化鱼油对鲤鱼生产性能和肌肉组织结构的影 响.动物营养学报,2001, 13(1):59-64]
[19] Hashimoto Y, Okaichi T.Muscli dystrophy of carp due to oxidized oil and the preventive effect of vitamin E [J].Bull Jpn Soc Sci Fish, 1966, 32(1):64-67
[20] Watanabe T, Matsuura Y.Eff ects of natural and synthetic antioxidants on the incidence of muscle dystrophy of carp induced by oxidized saury oil [J].Bull Jpn Soc Sci Fish, 1966, 2(10):887-891
[21] Aoe H, Saito T.Preventive effects of tocols on muscular dyst rophy of young carp [J].Bull Jpn Soc Sci Fish,1972, 38(8):845-851
[22] Chen A, Zhou M.The lipide preoxidateionwith diseases [J].Chinese Medical Journal,1985,5(11):704-706 [陈瑷, 周玫.脂质过氧化 作用与疾病.中国医学杂志, 1985,5(11):704-706]
[23] Vleet J F, Ferrans V J, Ruth G R.Ultrastructural alterations in nutritional cardiomyopathy of selenium-vitamin E deficient swine.1.Fiber lesions [J].Laboratory Investigation, 1977, 37(2): 188-200
[24] Vleet J F, Ferrans V J, Ruth G R.Ultrastructural alterations in nutritional cardiomyopathy of selenium-vitamin E deficient swine.2.Vascular lesions [J].Laboratory Investigation,1977, 37(2):201-211
[25] Nafstad I.Endothelial damage and platelet thrombosis associated with PUFA-rich, vitamin E deficient diet fed to pig [J].Thrombosis Research,1974, 5(2):251-258
[26] Zhang G Z, Cui Y N.The study on the pancreas islet αcel l function relation with selenium and the vitamin E [J].Chinese Pathologic Physiology Journal, 1999, 15(11):1027-1029 [张桂珍, 崔亚南. 胰岛α细胞机能与硒及维生素E 关系的研究.中国病理生理 杂志, 1999, 15(11):1027-1029]
计量
- 文章访问数: 898
- HTML全文浏览量: 0
- PDF下载量: 544
下载: