嗜水气单胞菌感染对背角无齿蚌5种免疫相关酶活力的影响

EFFECT OF LIGHTING,TEMPERATURE AND pH ON PHOTOSYNTHETIC CHARACTERS OF HAEMATOCOCCUS PLUVIALIS CG-11

    摘要
  • 摘要: 本研究以背角无齿蚌为材料,利用嗜水气单胞菌为诱导刺激物,对背角无齿蚌进行注射感染,在注射后3、6、12、24、48h分别取血淋巴、肝胰腺,测定其超氧化物歧化酶(SOD)、酚氧化酶(PO)、酸性磷酸酶(ACP)、碱性磷酸酶(AKP)及过氧化氢酶(CAT)的活力,并研究各项酶活力的变化规律。结果表明:除3h外,实验组肝胰腺的SOD活力均不同程度地高于对照组;血清实验组的PO活力开始显著高于对照组,然后降低,而肝胰腺实验组的PO活力持续高于对照组;另外,与对照组相比,实验组中血淋巴与肝胰腺的ACP、AKP和CAT活力在不同的时间段虽然有所增强,但两者之间无显著差异。因此,认为SOD、PO活性可以作为背角无齿蚌免疫抗病功能指标参数,而ACP、AKP及CAT活性能否作为该参数还有待于进一步研究。

     

    Abstract: The effects of light intensity, temperature and pH on photosynthetic characters of Haematococcus pluvialis CG-11 were studied by measuring photosynthetic O2 evolution and fluorescence kinetics. The results showed that the saturation thetic rate was obtained at 25℃; the photosynthetic efficiency kept quite high at the pH range of 7.5-8.0, with the maxi-range of 7.5-8.0, the Fv/Fm, Fv/Fo, ΦPSⅡ and ETR increased following the rising of pH, with their maximum values at pH 7.5, Fv/Fm and Fv/Fo decreased significantly over pH 7.5, while the decreasing trend didn't change obviously in ΦPSⅡ and ETR.

     

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