Abstract: The composihon of amino acids in muscle of juvenile (weight: 11-15g)one-year old(weight: 100-125g).two-year old(weight:220-225g) and three-year old(weight:450-480g) Chinese soft-shelled turtle (Trionyx sinensis) were analyzed: Theessential amino acids in muscle acount for 46.52% of total amino acids.The contentof glutamic acid in muscle is considerably high, reaching about 15% of tatal amino acids.On the basis of analysis and comparison, The nutritive value and specialmedical value of Chinese soft-shelled turtle are conffirmed and the theoretical groundsto develop the coordinating diet of Chinese soft-shelled turtle are provided in the paper.