鱼类病原菌中R+质粒的检出及其特性
DETECTION AND PROPERTIES OF TRANSFERABLE R+ PLASMID IN FISH-PATHOGENIC BACTERIA
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摘要: 本文研究了5个属的15株鱼类病原菌对17种抗菌药物的耐药性。其中,对β-内酰胺类和红霉素的耐药率最高,对妥布霉素最敏感。以E.coliK-12RC85为受体菌,鱼类病原菌为供体菌,采用细菌接合试验,从耐氨苄青霉素、四环素和磺胺的嗜水气单胞菌CJ26株中检测到一株耐四环素和磺胺的可自身传递的R质粒pWH9601。它对四环素的抗性可被四环素类药物所诱导。pWH9601上的耐药基因在供体菌和受体菌中的表达水平有所不同。该质粒可在37℃下,15min内通过接合方式,转移到E.coliK－12RC85中,而且这种转移在12℃的条件下也可进行。Abstract: Fifteen strains of fish pathogens were tested for their sensitivity to 17different antibacterial agents and the detected drug resistant strains were investigated todetermine if they possessed R plasmids.These pathogens included A.hydrophila,Vibrio, E.tarda, Yersinia and Pseudomonas.Most of them were resistant to β-Lactamsand erythromycin.All of tested strains were sensitive to tobramycin.The transferableR plasmid pWH9601 was detected from a strain of A.hydrophila CJ26.The detectedR plasmid showed resistance to tetracycline and sulfonamides.Resistance totetracycline conferred by pWH9601 was inducible in E.coli K-12 after exposure to1.0ug of tetracycline Per ml.This plasmid can transfer tO E.coli K-12 RC85 from A.hydrophila CJ26 within 0.25h at 37℃ by bacterial mating .This transfer can occur at12℃.
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Keywords:
- R plashed /
- Drug resistance /
- Fish pathogen
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