以精子为载体把外源DNA导入中华绒螯蟹的初步研究
PRELIMINARY STUDY ON SPERM AS VECTOR FOR INTRODUCING FOREIGN DNA INTO CHINESE MITTEN CRAB(ERIOCHEIR SINENSIS)
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Abstract: The study of crustacean genetics and breeding has been received more and more at-tention due to the rapid development of the shrimp and crab aquaculture.Chromosomesetmanipulation on shrimp and crab[1][2],and the work on transgenic shrimp[3][4] have gotsome progress.
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Keywords:
- Eriocheir sinensis /
- Foreign DNA /
- Sperm /
- Vector
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[1] Nan′er Cai, Lin Feng. Artificial induction of gynogenesis in Chinese shrimp Penaeusorientalis kishinouye[J].Aquaculture,1993,111:314[2] Lu Renhou, Zhang Jing, Liu Xiangu, et al. An experiment on induction of triploid in Eriocheir japonicus hepuensis Dai by heat treatment [J].Chinese jourmal of Oceanology and Limnology.1993,11(1),21-24[3] Kong Jie, Sun Xiaowen, Mai Ming, et al. Preliminary study on sperm cells as vectors for introducing foreign DNA into Penaeus chinensis [J].Marine Fisheries Research.1992,13,139-142.(in Chinese with English abstract)[4] Liu Ping, Kong Jie,Wang Qingyin et al. The studys on the Fertilized egg of Penaeus chinensis with microinjection of the growth hormone gene [J].Journal of Fishery Sciences of China.1996,3(4),35-39.(In Chinese with English abstract)[5] Sambrook J,Fritsch E F,Maniatis F.Molecular cloning: a laboratory manual(2nd)[M]New York: Cold Spring Harbor Laboratory Press.1989[6] Lavitrano M,Camqioni A,Faqio VM,et al. Sperm cells as vectors for introducing foreign DNA into eggs:genetic transformation of mice [J]. Cell.1989,57:717-723
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