鳗鲡开口病的病毒分离及特性研究
CHARACTERIZATION OF A TOGAVIRUS-LIKE AGENT,ISOLATED FROM EELS ANGUILLA JAPONIICA IN CHINA
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摘要: 从患开口病鳗中分离到一种病毒于30℃条件下,病毒能在EG细胞上产生细胞病变及形成1.2mm大小的空斑。电镜下观察,病毒呈圆形,具囊膜。膜上有纤状突起。成熟颗粒直径为70-85mm,其核酸为单链单分子RNA,病毒对氯仿,热和pH3不稳定,经多次冻融,其滴度稍有下降,病毒是在细胞浆内复制,温度范围在25-32℃之间,最适复制温度为30℃。Abstract: The present paper describes a new kind of epidemic which occurred in eels in South China in summer between temperatures ranging 25-32t and resulted in motality of more than 60% eels in some cultUre ponds. The diseased examination shows: Marked haemorrhage occurred in inter membrane of oral cavity and in cramal cavity and sometimes in the pectoral fin anal fin and abdomina surface.A new virus was isolated from the diseased eels, The virus was spherical in shape,40-80um in diameter, with an envelope tightly around a nucleus about 35-40um,sensitive to chloroform, labile to heat acid (PH3), freezing and thawing. The virus replicated successfully in eels gonads (EG) cell line at temperature 25-32℃ with optimal temperature at 30℃. However, CIK GCk, CAB, FHM and Epc lines were refractory to multiplication of this virus. which replicated in plasma of cells. The results suggest the tentative placement of the virus in the Family Togaviridae.
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Keywords:
- Isolation of eel virus /
- Characterization /
- Togavirus
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