草鱼GH单克隆抗体对9种硬骨鱼的免疫细胞化学定位
IMMUNOCYTOCHEMICAL LOCALIZATION OF NINE TELEOSTS USING MONOCLONAL ANTIBODY OF GRASS CARP GROWTH HORMONE
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摘要: 研究结果表明:1)鳜和鲇的胰岛中有大量的GH免疫活性内分泌细胞存在;8种有胃硬骨鱼的消化道中均未见到阳性特异性反应。2)草鱼的中腺、前腺垂体中均发现有GH和Som两种免疫活性内分泌细胞存在。还描述了胰岛和脑垂体中内分泌细胞的形态特征和分布特点并讨论了GH和Som这对具有相桔抗生理作用的抗原同时存在于草鱼垂体中的现象。Abstract: using immunocytochemical technique of Strept Avidin Biotin-peroxidaseComplex (SABC), growth hormone (GH) immunoreactive endocrine cells in thegastro-entero-pancreas of northern snakehead (Channa argus), largemouth bass(Micropterus salmoides), oriental sheatfish (Silurus asotus), yellow catfish (Pelteobagrusfulvidraco), mandarin fish (Siniperca chuatsi), ricefield eel (Monopterus albus),freshwater pomfret(Colossoma brachypomum), and nile tilapia (Oreochromis nilotica)were located with monoclonal antiserum of grass carp GH raised against mouse.Usingthe same method, immunocytochemical localization of the hypophysis of grass carpwere investigated with grass carp GH antiserum and somatostation (Som) antiserum.Results showed that many GH immunoreactive endocrine cells existed in the islets ofLangerhans of S.chuatsi and S. asotus, and no positive specificity specificity reaction was found in all digestive tract of ve tract of 8 teleosts GH- and Som-immunoreactive endocrine cells werefound in the proadenohypophysis and meso-adenohypophysis of grass carp.
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