青鱼肾脏(正常和患出血病)的组织学与超微结构的初步观察

PRELIMINARY OBSERVATION OF HISTOLOGICAL AND ULTRAMICROSCOPIC STRUCTURE ON THE KIDNEY (NORMAL AND HAEMORRHAGIC DISEASED) OF BLACK CARP (MYLOPHARYNGODON PICEUS)

  • 摘要: 本文报道了正常和患出血病的6~7月龄青鱼(Mylopharyngodon piceus)肾脏组织学光镜和电镜观察结果。在光镜下,青鱼的正常肾脏结构与草鱼等淡水鱼类极为相似。当青鱼患出血病时,肾脏的管腔上皮细胞变形,细胞核萎缩。此时,通过电镜观察,可以在细胞质内的线粒体中观察到大的空泡,同时在造血组织的包涵体中能清楚地看到病毒颗粒。病毒颗粒呈球形,直径为50—56毫微米,颗粒中央并具有一电子密度较高、直径为25—30毫微米的核心,在核心周围包有约16毫微米的外膜。

     

    Abstract: Both normal and artificially infected black carp aged 6—7 months were studied during 1982—1983. The structure of normal kidney tissue under optical microscope is similar to that of other freshwater fishes such as the grass carp. When the fish is infected by virus, the epithelial cells of segmental cavity in the kidney change in shape with atrophic nuclei. Under electron microscope, large vacuoles in intracytoplasmic mitochondria were observed. At the same time, many virus particles were found in inclusion bodies of hematopoietic tissues. The virus particles are spherical with diameter ranged 50—56nm. Each particle consists of an outer coat appoximately 16nm thick and a core with high electronic density measured 25—30 nm in diameter.

     

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