蛭弧菌Bdh522株理化特性和6S rDNA序列分析
THE PHYSIOLOGICAL CHARACTERISTICS AND 16S rDNA SEQUENCE ANALYSIS OF BDELLOVIBRIO SP1Bdh5221
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摘要: 蛭弧菌海水菌株Bdh522于2005年8月从海南琼海对虾养殖场周边排水沟水样中分离所得,具有裂解多数革兰氏阴细菌的特性。在前期Bdh522噬菌谱研究的基础上,利用其可利用热致死革兰氏阴细菌的生长特性,对其理化特性、药敏特性和6S rDNA序列分析进行了相关研究。结果表明:该株蛭弧菌为革兰氏阴性菌,呈杆状、弧状,一端有极长的极生鞭毛,细菌大小在(0.2-0.5)μm×(0.6-.5)μm左右;生化实验表明:Bdh522明胶穿刺试验和接触酶试验呈阳性,氧化酶反应呈阴性,对弧菌抑制剂O/29敏感;药敏实验表明:此菌对新霉素、卡那霉素、恩诺沙星敏感,对青霉素、磺胺嘧啶和万古霉素不敏感;对其6S rDNA序列的测定、分析表明,与其同源性最高的细菌是一株新发现的海洋新种细菌Kordiim onas gwangyangensis,同源性高达99%,而与其他蛭弧菌属细菌的同源性从90%到70%不等,并针对其基因序列做了相关进化分析。Abstract: A bacterium which isolated from prawn nursery wastewater in Qionghai,Hainan Province in August -005,was maintained by our laboratory and named Bdh5221.According to previous study,Bdh5221 could prey most Gram-negative bacterium and can also be cultivated by using heat-killed Gram-negative bacterium.In the present study,morphological,physiological characteristics,drug sensitivity,and 16S rDNA sequence of Bdh5221 were analyzed.The morphological studies showed that the strain Bdh5221 were Gram-negative,motile and had a bacilliformor bacilliform shape with flagellum at one side,which size was mostly(0.-- 0.5) μm ×(0.6- 1.5) μm.The physiological characteristics showed that this bacterium were catalase-positive,oxidase-negative,gelatin colliquation-positive and sensitive to the vibriostratic agent O/1-9.Drug sensitive results indicated that this bacterium were enrofloxacin-positive,neomgcin-positive,biograncetin-positive,penicillin-negative,Sulfadiazine-negative and vancocin-negative.16S rDNA gene of Bdellovibrio sp.Bdh5221 was 99% similar with a navel marine bacterium Kordiimonas gwangyangensis.The similarity with all recognized Bdellovibrio-and-like organisms was from 90% to 70%.It indicated that the bacterium might be a novel Bdellovibrio-and-like organisms.
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