Abstract: Two new species of trichodinids from skin, gills and mouth of freshwater fishes, Ctenogobius giurnus Retter, are described in this paper. One of them is Trichodina donghuensis sp. nov., body 49.2-60μm in disameter, adhesive disc 44.6(37.2-51.6)μm in diameter, denticulate ring 22.1(19.2-26.4)μm in diameter and its number of denticles from 22 to 28, 23-24 in most cases, with 8 radial pins each denticle and adoral zone about 410°, macronucleus horseshoe shaped, 20.4μm in out diameter and 4.8-5.4μm in length and 1.4μm in width. The another is Trichodina ctenogobii sp. nov., body 62.4-81.8μm in diameter, adhesive disc 63.9(56.4-72.0)μm in diameter, denticulate ring 33.3(31.8-38.4)μm in diameter and its number of denticles from 23 to 27 and with radial pins from 10 to 14, but 11 in most cases, adoral zone about 400°, macronucleus horseshoe shaped with different size of macronuclear arm and 42.0μm in out diameter, micronucleus oviform and situated at front side of left arm of macronucleus.