微囊藻毒素与自然水体中细菌VIVIFORM状态的相关性
THE STUDIES ON THE RELATIONSHIP BETWEEN MICROCYSTIN-LR AND VIVIFORM STATE OF BACTERIUM IN NATURAL BODIES OF WATER
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摘要: 细菌VIVIFORM状态是直接关系到自然水体水质评价和环境保护的生态现象，其形成机理相当复杂，但环境胁迫是主要诱因。通过人为改变湖水中的微囊藻毒素(MCLR)水平，对水体中蓝藻毒素与细菌VIVIFORM状态之间的相关性进行了分析。结果表明，较高的毒素水平对水体中细菌种群总量没有明显的影响，但能刺激VIVIFORM细菌转化成可培养状态，从而证实了自然水体中蓝藻毒素与水细菌VIVIFORM状态之间存在直接的相关性。
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关键词:
- 微囊藻毒素 /
- 细菌VIVIFORM状态 /
- 自然水体
Abstract: The bacterial viviform state is an important ecological problem which is relative to water quality accessment and environmental protection in natural water bodies. Though the reasons why it was formed are unclear and complicated,the direct effects came from the environmental stress factors including nutrient starvation,temperature changes and algal toxins stress etc. The rates of bacterial viviform states in different samping stations of Xili reservoir were investigated,the results showed: the bacteral total number was similar between the station of Dongjiang river entrance and the internal reseroir,but the rates of bacterial viviform state were lower in Dongjiang river entrance. It was deduced that environmental stress factors (such as high light intensity,water pollution and oxygen stress etc.)induced bacterial cell tybe transformation from culturable state to viviform cell. It was found that there were some relationship between blue-green bloom and bacterial viviform state in natural water bodies by studying on eutrophic water bodies in Lake Wenshanhu,Lake Xili and Lake Kinneret etc. The algal toxin is an important environmental factor in water bodies of blue-green bloom,the further experiments which studied on the influence of microcystins-LR on the viviform state of bacterium was done by using artificially induced microcystin-LR concentration gradients. The results showed microcystin-LR had no obvious effect on the aquatic bacterial population,but it could stimulated the bacterial cell transformation from viviform state to culturable state. It indicates that there is the direct relationship between microcystin-LR and the viviform state of bacterium in natural bodies of water. The further research on the molecular mechanism of microcystin-LR stimulating cell type transformation is onging.-
Keywords:
- Microcystin-LR /
- Viviform state of bacterium /
- Natural bodies of water
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[1] Xu H S,Roberts N,Singleton F,et al. Survival and viability of nonculturable Escherichia coli and Vibrio cholerae in the estuarine and marine environment [J].Microbiol. Ecol., 1982,8:313-323
[2] Xu B,Ji W S,Xu H S. An new bacteril survival state-Viable but nonculturable state[J]. Chinese Journal of Ecology, 1990,2(3):144-149 [许兵,纪伟尚,徐怀恕.一种新发现的细菌的特殊存活形式--活的非可培养状态.中国生态学杂志,1990,2(3):144-149]
[3] Porter K G,Feing Y S. The use of DAPi for identifying and counting aquatic microflora [J]. Limnol. Oceanogr.,1980,25(5):943-948
[4] Hu Z L,Bergstein T. The influence of artificially induced salinity changes on the bacterial population in Lake Kinneret [J]. Acta Hydrobiologrca Sinica,2000,24(4):326-330 [胡章立,Bergstein,T,人为因素导致的盐度变化对kinneret 湖水细菌种群的影响. 水生生物学报,2000,24(4):326-330]
[5] Mackintosh C,Beattie K A,Klunpp S,et al. Cyanobacterial microcystin-LR is a potent and specific inhibitor of protein phosphatase 1 and 2A from both mammals and higher plants [J]. FEBS Lett, 1990,264:187-192
[6] Palmer L. M. Molecular genetic and phenotypic alteration of Escherichia coli in natural water [J]. Microbial. Letters, 1984,21:169
[7] Mayra A.,William R,Nadathur S G. et al. Cultural and nonculturable bacterial symbionts in the toxic benthic dinoflagellate Ostreopsis lenticularis [J]. Toxicon, 2003,42:419-424
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